Department of Mechanical Engineering, Konkuk University, Seoul, 05029, Republic of Korea.
Lab Chip. 2018 Jun 26;18(13):1867-1874. doi: 10.1039/c8lc00382c.
A simple and effective platform that can conglomerate various microfluidic functions in a single chip is essential for many bioassays, especially for point-of-care testing applications. Here, a chip that exploits surface tension in solutions with movable top and bottom layers is presented, for use in fluid transport, mixing, maintaining metered volumes, and biomolecule capture and release. The chip has open chambers in vertically mobile top layers and rotationally mobile bottom layers to exploit surface tension in biochemical solutions, and implements control over fluid motion. To manipulate biomolecules, a vertically mobile tip with a permanent magnet at the top layer performs collection, transport, release, and dispersion of magnetic beads. Thus, the chip orchestrates various fluidic control functions without using on-chip valves and pumps that increase operational and structural complexity. To demonstrate its utility, the chip performs automated DNA extraction by obtaining genomic DNA from a sample containing cells. Our approach provides a useful and effective alternative to numerous platforms that use active and passive on-chip components for bioassays.
对于许多生物检测应用，特别是即时检测应用，一个能够将各种微流控功能集成在单个芯片上的简单有效的平台至关重要。在这里，我们提出了一种利用可移动顶层和底层溶液表面张力的芯片，用于流体传输、混合、保持计量体积以及生物分子的捕获和释放。该芯片在垂直移动的顶层和旋转移动的底层具有开放式腔室，以利用生化溶液中的表面张力，并实现对流体运动的控制。为了操纵生物分子，顶层带有永久磁铁的垂直移动尖端执行磁性珠的收集、输送、释放和分散。因此，该芯片无需使用增加操作和结构复杂性的片上阀门和泵，就可以协调各种流体控制功能。为了验证其用途，该芯片通过从含有细胞的样品中提取基因组 DNA 来实现自动化 DNA 提取。我们的方法为众多使用主动和被动片上组件进行生物检测的平台提供了一种有用且有效的替代方案。
