与 0.9%氯化钠相比，用 Plasma-Lyte A 洗涤血液成分可降低溶血并改善血小板功能。
Decreased Hemolysis and Improved Platelet Function in Blood Components Washed With Plasma-Lyte A Compared to 0.9% Sodium Chloride.
机构信息
Department of Pathology and Laboratory Medicine, Transfusion Medicine Division, Rochester, NY.
Departments of Environmental Medicine and Microbiology and Immunology, Rochester, NY.
出版信息
Am J Clin Pathol. 2018 Jul 3;150(2):146-153. doi: 10.1093/ajcp/aqy036.
OBJECTIVES
Washing cellular blood products is accepted to ameliorate repeated severe allergic reactions but is associated with RBC hemolysis and suboptimal platelet function. We compared in vitro hemolysis and platelet function in blood components after washing with Plasma-Lyte A (PL-A) vs normal saline (NS).
METHODS
RBC (n = 14) were washed/resuspended in NS or PL-A. Free hemoglobin and heme were determined at 0, 24, 48, and 72 hours. Platelet concentrates (PCs; n = 21) were washed with NS or PL-A and resuspended in same washing solution (n = 13) or ABO-identical plasma (n = 8). Platelet aggregation and spreading were evaluated.
RESULTS
The 24-hour free hemoglobin and heme levels were higher in NS (P < .05). Improved platelet function was observed in PL-A-washed PCs (P < .001).
DISCUSSION
PL-A showed less RBC hemolysis and better platelet function than NS. Whether such differences would occur in vivo is unknown.
目的
清洗细胞血液制品被认为可以改善反复发生的严重过敏反应，但与 RBC 溶血和血小板功能欠佳有关。我们比较了使用 Plasma-Lyte A（PL-A）和生理盐水（NS）洗涤后血液成分的体外溶血和血小板功能。
方法
将 RBC（n = 14）用 NS 或 PL-A 洗涤/重悬。在 0、24、48 和 72 小时时测定游离血红蛋白和血红素。用 NS 或 PL-A 洗涤血小板浓缩物（PC；n = 21），并分别在相同的洗涤液（n = 13）或 ABO 同型血浆（n = 8）中重悬。评估血小板聚集和铺展情况。
结果
NS 组在 24 小时时游离血红蛋白和血红素水平更高（P <.05）。PL-A 洗涤的 PC 血小板功能改善（P <.001）。
讨论
PL-A 引起的 RBC 溶血较少，血小板功能更好。尚不清楚这些差异是否会在体内发生。
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