Nelliat Mithun, Mohan Ganesh, Lukose Jijo, Shastry Shamee, Chidangil Santhosh
Centre of Excellence for Biophotonics, Department of Atomic and Molecular Physics, Manipal Academy of Higher Education, Manipal, India.
Department of Immunohematology and Blood Transfusion, Kasturba Medical College, Manipal Academy of Higher Education, Manipal, India.
Transfus Med Hemother. 2024 Jun 5;51(6):430-438. doi: 10.1159/000538972. eCollection 2024 Dec.
Raman tweezers spectroscopy (RTS) is a powerful tool that combines optical tweezers and Raman spectroscopy to study single living cells. RTS has become increasingly popular in biomedical and clinical research due to its high molecular specificity and sensitivity, which enable the study of cell viability, cell deformation, cell-protein, cell-nanoparticle, cell-cell interaction, etc. In transfusion medicine, RTS can give valuable insights into the storage lesions and effects of various preservatives and intravenous fluids on blood cells.
By analyzing the Raman spectra of individual blood cells, RTS can detect changes in the cellular blood components which can be used to monitor the quality of blood products during storage and transfusion. The present review article highlights the principle and clinical applications of RTS in transfusion medicine.
Raman spectroscopy is a versatile analytical method for biomedical research. Combining the Raman spectroscopy method with the optical tweezers technique will allow us to explore the dynamics of live single cells in their physiological medium.
拉曼镊子光谱（RTS）是一种强大的工具，它将光镊和拉曼光谱相结合，用于研究单个活细胞。由于其高分子特异性和灵敏度，RTS在生物医学和临床研究中越来越受欢迎，这使得能够研究细胞活力、细胞变形、细胞-蛋白质、细胞-纳米颗粒、细胞-细胞相互作用等。在输血医学中，RTS可以为各种防腐剂和静脉输液对血细胞的储存损伤和影响提供有价值的见解。
通过分析单个血细胞的拉曼光谱，RTS可以检测细胞血液成分的变化，这些变化可用于监测血液制品在储存和输血过程中的质量。本综述文章重点介绍了RTS在输血医学中的原理和临床应用。
拉曼光谱是生物医学研究中一种多功能的分析方法。将拉曼光谱方法与光镊技术相结合，将使我们能够在其生理介质中探索活的单个细胞的动态。
