Urbanowski M L, Stauffer G V
Nucleic Acids Res. 1985 Feb 11;13(3):673-85. doi: 10.1093/nar/13.3.673.
The nucleotide sequence of the Salmonella typhimurium metJ gene is presented along with the sequence of the promoter region for the closely linked metB gene. The two genes are transcribed in opposite directions, with transcription initiating from a single promoter for metB, and from two apparent promoters for metJ. RNA polymerase binding sites for metJ and metB, determined by in vitro protection studies, lie adjacent to each other and may overlap. The two metJ promoters, PJ1 and PJ2, are separated by approximately 65 base pairs. Binding of RNA polymerase in vitro could only be observed for PJ1, even though transcripts are initiated from both promoters in vivo. The metJ gene codes for a polypeptide of 105 amino acids with a calculated Mr of 12,110. The translation start site was determined by N-terminal amino acid sequence analysis of a metJ-lacZ fusion protein.
本文给出了鼠伤寒沙门氏菌metJ基因的核苷酸序列以及紧密连锁的metB基因启动子区域的序列。这两个基因转录方向相反，metB基因从单一启动子起始转录，而metJ基因从两个明显的启动子起始转录。通过体外保护研究确定的metJ和metB的RNA聚合酶结合位点彼此相邻且可能重叠。两个metJ启动子PJ1和PJ2相隔约65个碱基对。尽管体内转录从两个启动子起始，但体外仅能观察到RNA聚合酶与PJ1结合。metJ基因编码一个由105个氨基酸组成的多肽，计算的Mr为12,110。通过对metJ - lacZ融合蛋白的N端氨基酸序列分析确定了翻译起始位点。
