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大肠杆菌K-12大细胞制备物中metF基因表达的调控：metJ蛋白的可逆作用及维生素B12的影响

Control of metF gene expression in maxicell preparations of Escherichia coli K-12: reversible action of the metJ protein and effect of vitamin B12.

作者信息

Emmett M R, Johnson J R

出版信息

J Bacteriol. 1986 Dec;168(3):1491-4. doi: 10.1128/jb.168.3.1491-1494.1986.

DOI:10.1128/jb.168.3.1491-1494.1986
PMID:3536888
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC213670/
Abstract

Expression of methionine regulon elements was controlled by the metJ protein gpMetJ. A maxicell system with cloned copies of the metF transcription unit allowed reversible action of gpMetJ. Expression of the metF transcription unit in maxicells was reduced by exogenous vitamin B12 at concentrations of 0.5 nM or greater.

摘要

甲硫氨酸调节子元件的表达受metJ蛋白gpMetJ的控制。带有metF转录单位克隆拷贝的大细胞系统可实现gpMetJ的可逆作用。在大细胞中，当外源维生素B12浓度达到0.5 nM或更高时，metF转录单位的表达会降低。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7fc6/213670/b9642070cd09/jbacter00205-0452-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7fc6/213670/b6b16f68f875/jbacter00205-0452-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7fc6/213670/b9642070cd09/jbacter00205-0452-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7fc6/213670/b6b16f68f875/jbacter00205-0452-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7fc6/213670/b9642070cd09/jbacter00205-0452-b.jpg

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本文引用的文献

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Regulation of methionine synthesis in Escherichia coli: Effect of metJ gene product and S-adenosylmethionine on the expression of the metF gene.大肠杆菌甲硫氨酸合成的调控：metJ 基因产物和 S-腺苷甲硫氨酸对 metF 基因表达的影响。
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The Escherichia coli K-12 metJ193 allele contains a point mutation which alters the hydrophobic pocket responsible for in vitro binding of S-adenosylmethionine: effects on cell growth and induction of met regulon expression.大肠杆菌K-12 metJ193等位基因包含一个点突变，该突变改变了负责体外结合S-腺苷甲硫氨酸的疏水口袋：对细胞生长和甲硫氨酸操纵子表达诱导的影响。
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Nucleotide sequence of metF, the E. coli structural gene for 5-10 methylene tetrahydrofolate reductase and of its control region.大肠杆菌5-10亚甲基四氢叶酸还原酶结构基因metF及其调控区的核苷酸序列。
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