Many musicians suffer from playing-related musculoskeletal disorders (PRMDs) however many of the epidemiological surveys on PRMD do not utilize evaluated self-report instruments, especially as a tool to assess musculoskeletal pain intensity and pain interference in terms of function and psychosocial constructs. The aim of this study was to translate and cross-culturally adapt the Musculoskeletal Pain Intensity and Interference Questionnaire for Musicians (MPIIQM) into German, and validate it among a population of professional orchestral musicians. The translated and cross-culturally adapted German version of the questionnaire was sent to a total of 367 professional orchestral musicians in Germany who were eligible for the study. Of this total, 124 musicians responded to the questionnaire. The psychometric evaluation showed a clear two dimensionality namely "pain intensity" and "pain interference". Internal consistency was very high for both the "pain intensity" and "pain interference" dimensions as well as for the complete scale. The values for the Intraclass Correlation Coefficient for the test-retest reliability indicated good to excellent repeatability. The subscales of MPIIQM showed strong correlation with the Brief Pain Inventory (BPI) and the shorter version of Disabilities of the Arm, Shoulder and Hand (QuickDash) and low correlation with the Fatigue Severity Scale (FSS). These results were as expected, confirming the good construct validity of the MPIIQM. The German Version of the MPIIQM (MPIIQM-G) and the original version have similarly high qualities and thus the MPIIQM-G represents a useful self-report instrument for the measurement and evaluation of musculoskeletal pain intensity and pain interference for professional orchestral musicians.