Lechner J F, Tokiwa T, LaVeck M, Benedict W F, Banks-Schlegel S, Yeager H, Banerjee A, Harris C C
Proc Natl Acad Sci U S A. 1985 Jun;82(11):3884-8. doi: 10.1073/pnas.82.11.3884.
Replicative cultures of human pleural mesothelial cells were established from noncancerous adult donors. The cells exhibited normal mesothelial cell characteristics including keratin, hyaluronic acid mucin, and long branched microvilli, and they retained the normal human karyotype until senescence. The mesothelial cells were 10 and 100 times more sensitive to the cytotoxic effects of asbestos fibers than normal human bronchial epithelial or fibroblastic cells, respectively. In addition, cultures of mesothelial cells that survived two cytotoxic exposures of amosite fibers were aneuploid with consistent specific chromosomal losses indicative of clonal origin. These aneuploid cells exhibit both altered growth control properties and a population doubling potential of greater than 50 divisions beyond the culture life span (30 doublings) of the control cells.
人胸膜间皮细胞的复制性培养物取自无癌成年供体。这些细胞表现出正常间皮细胞特征，包括角蛋白、透明质酸粘蛋白和长分支微绒毛，并且在衰老前保持正常人类核型。间皮细胞对石棉纤维细胞毒性作用的敏感性分别比正常人支气管上皮细胞或成纤维细胞高10倍和100倍。此外，在两次铁石棉纤维细胞毒性暴露中存活下来的间皮细胞培养物为非整倍体，具有一致的特定染色体缺失，表明其克隆起源。这些非整倍体细胞既表现出改变的生长控制特性，又具有超过对照细胞培养寿命（30次倍增）50次以上分裂的群体倍增潜力。
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