三（1-氯-2-丙基）磷酸酯（TCPP）对贻贝 Mytilus galloprovincialis 免疫的生物学效应。
Biological effects of tris (1-chloro-2-propyl) phosphate (TCPP) on immunity in mussel Mytilus galloprovincialis.
机构信息
Key Laboratory of Coastal Zone Environmental Processes and Ecological Remediation, Yantai Institute of Coastal Zone Research (YIC), Chinese Academy of Sciences(CAS), Shandong Provincial Key Laboratory of Coastal Zone Environmental Processes, YICCAS, Yantai, 264003, PR China; Laboratory for Marine Fisheries Science and Food Production Processes, Qingdao National Laboratory for Marine Science and Technology, Qingdao, 266237, PR China.
Key Laboratory of Coastal Zone Environmental Processes and Ecological Remediation, Yantai Institute of Coastal Zone Research (YIC), Chinese Academy of Sciences(CAS), Shandong Provincial Key Laboratory of Coastal Zone Environmental Processes, YICCAS, Yantai, 264003, PR China; University of Chinese Academy of Sciences, Beijing, 100049, PR China.
出版信息
Environ Toxicol Pharmacol. 2018 Jul;61:102-106. doi: 10.1016/j.etap.2018.05.022. Epub 2018 May 31.
Organophosphate flame retardants (OPFRs) are increasingly produced and used as alternatives of brominated flame-retardants (BFRs) and have become emerging marine environmental contaminants. So far, however, little is known regarding the biological effects of OPFRs in marine organisms. In this study, the biological effects of one of the most abundant OPFRs, tris (1-chloro-2-propyl) phosphate (TCPP), on the immunity in mussel Mytilus galloprovincialis were characterized by testing the reactive oxygen species, apoptosis, antioxidant system and immunity related gene expressions. Results indicated that both TCPP exposures (10 and 100 nmol L) significantly (p < 0.01) enhanced reactive oxygen species production and the high dose of TCPP induced more apoptosis and oxidative stress in mussel hemocytes. TCPP also induced an obvious hormesis phenomenon (low dose inhibition and high dose stimulation) in mussel hemocytes, as indicated by the gene expression profiles of caspase 8 and mytimacin. The down-regulated gene expression levels of lysozymes suggested that both TCPP exposures inhibited the innate immunity in mussel M. galloprovincialis. The significantly (p < 0.01) increased gene expression levels of TLR, galectin, PGRP and LITAF demonstrated that TCPP induced dose-dependent immune stress in mussels. Overall, this work suggested that TCPP could influence the immune system in marine mussel M. galloprovincialis.
有机磷阻燃剂（OPFRs）作为溴化阻燃剂（BFRs）的替代品被越来越多地生产和使用，并已成为新兴的海洋环境污染物。然而，到目前为止，人们对海洋生物中 OPFRs 的生物效应知之甚少。在这项研究中，通过测试活性氧、细胞凋亡、抗氧化系统和免疫相关基因表达，研究了最丰富的 OPFR 之一三（1-氯-2-丙基）磷酸酯（TCPP）对贻贝 Mytilus galloprovincialis 免疫力的生物学影响。结果表明，TCPP 暴露（10 和 100nmol/L）均显著（p<0.01）增加了活性氧的产生，高剂量的 TCPP 诱导贻贝血细胞更多的细胞凋亡和氧化应激。TCPP 还在贻贝血细胞中诱导了明显的毒物兴奋效应（低剂量抑制和高剂量刺激），这表现在半胱天冬酶 8 和 mytimacin 的基因表达谱上。溶菌酶的基因表达水平下调表明，TCPP 抑制了贻贝 M. galloprovincialis 的先天免疫。TLR、半乳糖凝集素、PGRP 和 LITAF 的基因表达水平显著（p<0.01）增加表明，TCPP 诱导了贻贝的剂量依赖性免疫应激。总的来说，这项工作表明 TCPP 可能会影响海洋贻贝 M. galloprovincialis 的免疫系统。
Zotero 插件