Uporova T M, Kartashova I M, Skripkin E A, Lopareva E n, Nikol'skaia I I
Vopr Med Khim. 1985 Mar-Apr;31(2):131-6.
Two restrictases SsoI and SsoII, belonging to the enzymes of restriction of the class II, were isolated from a strain of dysenteric bacteria. The structure of the site sensitive to SsoI and SsoII was studied after fragmentation of testor DNA as well as by means of direct determination of nucleotide sequence. SsoI was shown to be an isoschizomer of the EcoRI restrictase from E. coli. Restrictase SsoII proved to be a new enzyme, which hydrolyzed the sequence 5' ...CCNGG.. 3' and was distinct from the known restrictases as shown by studies of the type of DNA hydrolyzed. A three-step procedure is developed for isolation of SsoII restrictase involving the consecutive chromatography on Blue Sepharose, phosphocellulose PII and phenyl-Sepharose. Restrictase SsoI and EcoRI were isolated by means of isoelectrofocusing using ampholines.
从一株痢疾杆菌中分离出了两种属于II类限制酶的限制酶SsoI和SsoII。在对测试DNA进行片段化后，以及通过直接测定核苷酸序列的方法，研究了对SsoI和SsoII敏感的位点结构。结果表明，SsoI是来自大肠杆菌的EcoRI限制酶的同裂酶。限制酶SsoII被证明是一种新酶，它能水解5'...CCNGG.. 3'序列，并且从所水解的DNA类型研究来看，它与已知的限制酶不同。开发了一种三步法来分离SsoII限制酶，包括依次在蓝色琼脂糖凝胶、磷酸纤维素PII和苯基琼脂糖凝胶上进行色谱分离。通过使用两性电解质进行等电聚焦的方法分离出了限制酶SsoI和EcoRI。
