[来自链霉菌属菌株的限制性内切酶Sst 12I，识别核苷酸序列5'-CTGCAG-3']
[Restriction endonuclease Sst 12I from a strain of Streptomyces sp. recognizing the nucleotide sequence 5'-CTGCAG-3'].
作者信息
Ushakova T A, Puchkova L I, Gutorov V V, Kuvshinov V N, Repin V I
机构信息
State Research Center of Virology and Biotechnology Vector, Koltsovo, Novosibirsk Oblast, 630559 Russia.
出版信息
Prikl Biokhim Mikrobiol. 2002 Jan-Feb;38(1):25-8.
A new restriction endonuclease Sst12I belonging to the II type and recognizing the sequence 5'-CTGCAG-3' was isolated from the bacterial strain Streptomyces sp. St-12. The enzyme hydrolyzes DNA between adenine and guanine residues; thus, it is a true isoschizomer of restrictase PstI. In contrast to PstI, the restriction endonuclease Sst12I hydrolyses DNA both at 37 degrees and 55 degrees C and remains active after long-term storage.
从链霉菌属细菌菌株St-12中分离出一种新的II型限制性内切酶Sst12I，其识别序列为5'-CTGCAG-3'。该酶在腺嘌呤和鸟嘌呤残基之间水解DNA；因此，它是限制性酶PstI的真正同裂酶。与PstI不同的是，限制性内切酶Sst12I在37℃和55℃时均能水解DNA，并且在长期保存后仍保持活性。
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