Stiller-Winkler R, Stark M, Idel H
Zentralbl Bakteriol Mikrobiol Hyg B. 1985 Mar;180(4):381-93.
209 sera of tumor-bearing patients and 133 control sera were tested by anti-complement immunofluorescence (ACIF) on 2 xenotransplants of human bronchogenic cancer and on 2 xenotransplants of rectum carcinoma passaged in nu/nu mice. Testing of sera from patients with bronchogenic carcinoma resulted in a positive reaction with the xenotransplants of bronchogenic cancer in 48.5 to 58.5%, and sera of patients with digestive tract cancer in 6.6 to 16.1%. On xenotransplants of rectum carcinoma 73.2 to 78.1% of the sera from patients with bronchogenic carcinomas and 22.6 to 29.1% of the sera of patients with digestive tract cancer reacted positively. Control sera were positive dependent on the xenotransplants in 2.0 to 12.5%. These results indicate that there are antibodies in the sera of tumor-bearing patients against one or more non organ specific tumor-associated antigen(s) occurring in an elevated concentration in carcinomas of the digestive tract than in bronchogenic carcinomas. After absorption of the sera with homogenates of bronchogenic carcinoma and liver metastases of colon carcinoma the reaction was inhibited in the ACIF, reaching control values.
采用抗补体免疫荧光法（ACIF），对209份荷瘤患者血清和133份对照血清在2株人支气管癌异种移植瘤以及2株在裸鼠体内传代的直肠癌异种移植瘤上进行检测。对支气管癌患者血清的检测结果显示，与支气管癌异种移植瘤出现阳性反应的比例为48.5%至58.5%，消化道癌患者血清的阳性反应比例为6.6%至16.1%。在直肠癌异种移植瘤上，支气管癌患者血清的阳性反应比例为73.2%至78.1%，消化道癌患者血清的阳性反应比例为22.6%至29.1%。对照血清的阳性反应率因异种移植瘤不同在2.0%至12.5%之间。这些结果表明，荷瘤患者血清中存在针对一种或多种非器官特异性肿瘤相关抗原的抗体，这些抗原在消化道癌中的浓度高于支气管癌。用支气管癌匀浆和结肠癌肝转移灶匀浆吸收血清后，ACIF反应受到抑制，达到对照值。
