Laboratoire de Biologie et Physiologie des Organismes (LBPO), Faculté des Sciences Biologiques, Université des Sciences et de la Technologie Houari Boumediene (USTHB), Bab Ezzouar, Algérie.
Institut de Génétique Moléculaire de Montpellier, IGMM, CNRS, Université de Montpellier, Montpellier, France.
Environ Technol. 2019 Dec;40(28):3713-3723. doi: 10.1080/09593330.2018.1485752. Epub 2018 Jun 13.
The aim of this work was to determinate effect of naphthalene at different concentrations on morphological, physiological and some metabolic responses of pea seedlings. The quantification of naphthalene and its by-products were also recorded by Gas Chromatography/ Mass Spectrometry (GC / MS) in soil and in the different plant parts (roots, stems, leaves and fruit). In our controlled laboratory studies, plants exposed to naphthalene were able to efficiently grow and maintain their content of chlorophyll and carotenoids comparatively to the control plants. However, the pollutant slightly increased the amounts of fatty acid peroxides and strongly those of malonyldialdehyde, the product of lipid peroxidation. The glutathione S transferase activity was also increased for all concentrations used especially in leaves. Chromatograms showed that naphthalene has fallen sharply in the soil or even disappeared for the highest concentration from the second to third week. Furthermore, the removal ratio of 67% of the pollutant from the soil was distributed between two metabolites (ion 47 and ion 59) in the leaves for this same concentration in only three weeks of cultivation. In parallel, the amount of pollutant remained higher in unvegetated control soil. These results suggest that seedlings of pea ( L.) can remove naphthalene from contaminated soil and consequently have a high potential to be used as a promising candidate for the phytoremediation of naphthalene-contaminated soil.
本研究的目的是测定不同浓度萘对豌豆幼苗形态、生理和某些代谢反应的影响。通过气相色谱/质谱（GC/MS）还记录了萘及其副产物在土壤和不同植物部分（根、茎、叶和果实）中的定量。在我们的实验室控制研究中，暴露于萘的植物能够有效地生长并维持其叶绿素和类胡萝卜素的含量与对照植物相当。然而，污染物略微增加了脂肪酸过氧化物的含量，强烈增加了丙二醛的含量，丙二醛是脂质过氧化的产物。谷胱甘肽 S 转移酶的活性也增加了，所有使用的浓度，特别是在叶片中。色谱图显示，萘在第二至第三周从土壤中急剧下降，甚至在最高浓度下消失。此外，在仅三周的培养时间内，同一浓度下，污染物的去除率为 67%，从土壤中分布在两种代谢物（离子 47 和离子 59）之间。同时，未植被对照土壤中污染物的含量仍然较高。这些结果表明，豌豆（L.）幼苗可以从污染的土壤中去除萘，因此具有作为萘污染土壤植物修复的有前途候选物的高潜力。
