An assay method for cyclic AMP using high-performance liquid chromatography with pretreatment by alkaline phosphatase.

A convenient method for determination of cyclic AMP is described. This nucleotide was determined using high-performance liquid chromatography after the treatment of tissue extracts by alkaline phosphatase. Tissue extracts contain large amounts of materials which interfere with the measurement of cyclic AMP on the chromatogram. By the alkaline phosphatase treatment, these materials were completely converted to compounds which no longer interfered with the measurement. This method enables the detection of 2 pmol cyclic AMP, and is applicable to various tissue extracts which contain at least 0.1 nmol cyclic AMP/g wet wt.