Departament de Química Analítica i Química Orgànica, Universitat Rovira i Virgili (URV), C/Marcel·lí Domingo 1, 43007 Tarragona, Spain.
Departament de Química Analítica i Química Orgànica, Universitat Rovira i Virgili (URV), C/Marcel·lí Domingo 1, 43007 Tarragona, Spain.
Food Chem. 2018 Nov 1;265:64-69. doi: 10.1016/j.foodchem.2018.05.082. Epub 2018 May 18.
A new biosensor for monitoring glucose levels in beverages is presented. The measurements are performed using potentiometric detection. Working electrodes are made using platinised paper as support and a biocompatible polymeric membrane made of a mixture of polyvinyl alcohol and chitosan containing glucose oxidase as the recognition layer. The system is based on the detection of the hydrogen peroxide generated by an enzymatic reaction performed in a highly sensitive, selective and simple way. The biosensors display suitable analytical performance (sensitivity -119.6 ± 6.4 mV/dec in the 0.03-1.0 mM range with a limit of detection of 0.02 mM). Determination of glucose in commercial orange juices is presented. These results were validated against conventional standard methods, showing good accuracy and fast analytical response. The methodology presented herein does not require complex samples treatment, offering an alternative to conventional methods, particularly for determinations performed with minimal expertise and without a laboratory infrastructure.
本文提出了一种用于监测饮料中葡萄糖水平的新型生物传感器。该测量采用电位检测法进行。工作电极使用镀铂纸作为支撑，并使用包含葡萄糖氧化酶的聚乙烯醇和壳聚糖混合物制成的生物相容性聚合物膜作为识别层。该系统基于以高度灵敏、选择性和简单的方式进行的酶反应所产生的过氧化氢的检测。生物传感器显示出合适的分析性能（在 0.03-1.0 mM 范围内的灵敏度为-119.6 ± 6.4 mV/dec，检测限为 0.02 mM）。本文介绍了商业橙汁中葡萄糖的测定。这些结果通过与传统标准方法进行了验证，显示出良好的准确性和快速的分析响应。本文提出的方法不需要复杂的样品处理，为传统方法提供了一种替代方法，特别是对于具有最小专业知识和没有实验室基础设施的情况下进行的测定。
