Akima K, Yoshioka M, Imai C, Horiba M, Honma T, Ishitani K
Gan To Kagaku Ryoho. 1985 Jun;12(6):1278-85.
Schizophyllan (SPG) was administered to 13 lung cancer patients (i.m. 20mg X 2/week) for 3 weeks without chemo or irradiation therapies, and serum proteins were analyzed by two-dimensional electrophoresis (TDE). Additionally, immunosuppressive acidic protein (IAP) was quantitatively determined by single radial immunodiffusion (SRID). By TDE analysis, human serum proteins were separated into more than 100 spots, and about 14 spots were found to show quantitative changes in cancer patients. Quantitative examination was therefore conducted on changes of 8 components among these spots, including alpha 1-acidic glycoprotein (alpha 1 AG), acidic alpha 2-macroglobulin (acidic alpha 2 M), haptoglobin (Hp) and IAP. The protein which showed the most marked decrease in cancer patients, located between transferrin and IgG on the above TDE patterns, was ascertained to have a molecular weight of about 150,000 using a gel filtration method. This protein was increased in 7 of 13 patients after SPG treatment.
对13名肺癌患者进行裂褶多糖（SPG）治疗（肌肉注射，20毫克×每周2次，共3周），期间不进行化疗或放疗，采用双向电泳（TDE）分析血清蛋白。此外，通过单向放射免疫扩散法（SRID）对免疫抑制酸性蛋白（IAP）进行定量测定。通过TDE分析，人血清蛋白被分离成100多个斑点，发现癌症患者中约有14个斑点显示出定量变化。因此，对这些斑点中的8种成分的变化进行了定量检测，包括α1-酸性糖蛋白（α1AG）、酸性α2-巨球蛋白（酸性α2M）、触珠蛋白（Hp）和IAP。在上述TDE图谱中，位于转铁蛋白和IgG之间、在癌症患者中下降最明显的蛋白质，采用凝胶过滤法确定其分子量约为150,000。13名患者中有7名在SPG治疗后该蛋白质增加。
