Cammer W, Sacchi R, Kahn S
Brain Res. 1985 May;352(1):89-96. doi: 10.1016/0165-3806(85)90090-2.
Rat central nervous system (CNS) tissue sections were immunostained by the peroxidase-anti-peroxidase (PAP) method using a rabbit serum directed against rat liver 5'-nucleotidase. In paraffin sections from the brains of 60-day-old rats 5'-nucleotidase immunoreactivity occurred in the same white-matter regions as myelin-basic protein immunoreactivity and histological staining of myelin. The immunostaining of cerebral white matter for 5'-nucleotidase was more intense and wide-spread at the age of 120 days than at 60 days, and the choroid plexus and blood vessels were stained consistently. In the paraffin sections from the brains of younger (20-day-old) rats the staining of 5'-nucleotidase in the white matter was faint and patchy. In paraffin sections from spinal cord, 5'-nucleotidase immunoreactivity was observed throughout the lateral white-matter columns and, frequently, in the cell bodies of interfascicular oligodendroglia. Interfascicular oligodendroglia also showed 5'-nucleotidase immunoreactivity in vibratome sections from the CNS tissue of young and adult rats. The findings were consistent with histochemical and biochemical evidence for 5'-nucleotidase in rat brain myelin and oligodendroglia, with substantial increases in activity in the myelin as rats develop from the ages of 20 to 120 days. 5'-Nucleotidase immunoreactivity was not observed in any astrocytes or in oligodendrocytes in the gray matter; however, the enzyme may occur in those glial cells at levels lower than were detectable using the present method.
采用抗大鼠肝脏5'-核苷酸酶的兔血清，通过过氧化物酶-抗过氧化物酶（PAP）法对大鼠中枢神经系统（CNS）组织切片进行免疫染色。在60日龄大鼠大脑的石蜡切片中，5'-核苷酸酶免疫反应性出现在与髓鞘碱性蛋白免疫反应性以及髓鞘组织学染色相同的白质区域。120日龄时大脑白质的5'-核苷酸酶免疫染色比60日龄时更强烈且分布更广，脉络丛和血管持续被染色。在年幼（20日龄）大鼠大脑的石蜡切片中，白质中5'-核苷酸酶的染色微弱且呈斑片状。在脊髓的石蜡切片中，在整个外侧白质柱中观察到5'-核苷酸酶免疫反应性，并且在束间少突胶质细胞的细胞体中也经常观察到。在幼龄和成年大鼠CNS组织的振动切片中，束间少突胶质细胞也显示出5'-核苷酸酶免疫反应性。这些发现与大鼠脑髓鞘和少突胶质细胞中5'-核苷酸酶的组织化学和生化证据一致，随着大鼠从20日龄发育到120日龄，髓鞘中的活性大幅增加。在任何星形胶质细胞或灰质中的少突胶质细胞中均未观察到5'-核苷酸酶免疫反应性；然而，该酶可能以低于本方法可检测水平存在于那些神经胶质细胞中。
