Shahrestani Morteza, Tehrani Mohammad Saber, Shoeibi Shahram, Aberoomand Azar Parviz, Waqif Husain Syed
Department of Analytical Chemistry, Faculty of Basic Science, Science and Research Branch, Islamic Azad University, Tehran, Iran.
Food and Drug Laboratories Research Center (FDLRC), Iran Food and Drug Administration (IFDA), MOH, Tehran, Iran.
J Anal Methods Chem. 2018 May 15;2018:1651629. doi: 10.1155/2018/1651629. eCollection 2018.
The primary aromatic amines (PAAs) are food contaminants which may exist in packaged food. Polyurethane (PU) adhesives which are used in flexible packaging are the main source of PAAs. It is the unreacted diisocyanates which in fact migrate to foodstuff and then hydrolyze to PAAs. These PAAs include toluenediamines (TDAs) and methylenedianilines (MDAs), and the selected PAAs were 2,4-TDA, 2,6-TDA, 4,4'-MDA, 2,4'-MDA, and 2,2'-MDA. PAAs have genotoxic, carcinogenic, and allergenic effects. In this study, extraction methods were applied on a 3% acetic acid as food simulant which was spiked with the PAAs under study. Extraction methods were liquid-liquid extraction (LLE), dispersive liquid-liquid microextraction (DLLME), and solid-phase extraction (SPE) with C18 ec (octadecyl), HR-P (styrene/divinylbenzene), and SCX (strong cationic exchange) cartridges. Extracted samples were detected and analyzed by HPLC-UV. In comparison between methods, recovery rate of SCX cartridge showed the best adsorption, up to 91% for polar PAAs (TDAs and MDAs). The interested PAAs are polar and relatively soluble in water, so a cartridge with cationic exchange properties has the best absorption and consequently the best recoveries.
伯芳香胺（PAAs）是可能存在于包装食品中的食品污染物。用于软包装的聚氨酯（PU）粘合剂是PAAs的主要来源。实际上是未反应的二异氰酸酯迁移到食品中，然后水解为PAAs。这些PAAs包括甲苯二胺（TDAs）和亚甲基二苯胺（MDAs），所选择的PAAs为2,4 - TDA、2,6 - TDA、4,4'- MDA、2,4'- MDA和2,2'- MDA。PAAs具有遗传毒性、致癌性和致敏性。在本研究中，提取方法应用于添加了所研究PAAs的3%乙酸作为食品模拟物。提取方法有液 - 液萃取（LLE）、分散液 - 液微萃取（DLLME）以及使用C18 ec（十八烷基）、HR - P（苯乙烯/二乙烯基苯）和SCX（强阳离子交换）柱的固相萃取（SPE）。提取的样品通过高效液相色谱 - 紫外检测法进行检测和分析。在方法比较中，SCX柱的回收率显示出最佳吸附效果，对于极性PAAs（TDAs和MDAs）高达91%。所关注的PAAs具有极性且相对易溶于水，因此具有阳离子交换特性的柱具有最佳吸附效果，从而回收率也最佳。
