Werns S W, Shea M J, Driscoll E M, Cohen C, Abrams G D, Pitt B, Lucchesi B R
Circ Res. 1985 Jun;56(6):895-8. doi: 10.1161/01.res.56.6.895.
Previous studies demonstrated a significant reduction of ultimate infarct size in the canine heart by the combined administration of superoxide dismutase plus catalase. This study was performed to assess the independent effects of each enzyme on ultimate infarct size due to ischemia/reperfusion. Dogs received 2-hour infusions of superoxide dismutase, catalase, or albumin (controls) via the left atrium beginning 15 minutes before and ending 15 minutes after a 90-minute occlusion of the left circumflex coronary artery. The dogs were killed 6 hours after reperfusion. After histochemical staining, infarct and risk area masses were calculated by gravimetric and planimetric analysis. Infarct size expressed as a percentage of the area at risk was: superoxide dismutase, 19 +/- 5; catalase, 30 +/- 5; and controls, 40 +/- 3. Infarct size in the superoxide dismutase group, but not the catalase group, was significantly less than in controls (P less than 0.05). No significant differences in hemodynamics or area at risk were observed that could explain the differences in infarct size. The results indicate that superoxide dismutase alone protects reperfused ischemic myocardium as well as does the combination of superoxide dismutase and catalase. The beneficial effect of superoxide dismutase and insignificant effect of catalase suggest that tissue damage during ischemia and reperfusion may be mediated largely by superoxide anion but not by hydrogen peroxide.
先前的研究表明，超氧化物歧化酶与过氧化氢酶联合给药可显著减小犬心脏最终梗死面积。本研究旨在评估每种酶对缺血/再灌注所致最终梗死面积的独立作用。在左旋冠状动脉闭塞90分钟前15分钟开始，至闭塞后15分钟结束，通过左心房给犬输注超氧化物歧化酶、过氧化氢酶或白蛋白（对照组）2小时。再灌注6小时后处死犬。组织化学染色后，通过重量法和平面测量法计算梗死和危险区域的质量。梗死面积占危险区域面积的百分比为：超氧化物歧化酶组19±5；过氧化氢酶组30±5；对照组40±3。超氧化物歧化酶组的梗死面积显著小于对照组（P<0.05），而过氧化氢酶组则不然。未观察到能解释梗死面积差异的血流动力学或危险区域面积的显著差异。结果表明，单独使用超氧化物歧化酶对再灌注的缺血心肌的保护作用与超氧化物歧化酶和过氧化氢酶联合使用时相同。超氧化物歧化酶的有益作用和过氧化氢酶的无显著作用表明，缺血和再灌注期间的组织损伤可能主要由超氧阴离子介导，而非由过氧化氢介导。
