Xie Lu-Shuang, Zhao Na, Yang Hui, Huang Biao, Tang Yong, Wu Qiao-Feng, Jing Xiang-Hong, Yu Shu-Guang
College of Acupuncture and Moxibustion, Chengdu University of Traditional Chinese Medicine, Chengdu 610075, China; Institute of Acupuncture-moxibustion and Homeostasis Regulation, Chengdu University of Traditional Chinese Medicine, Chengdu 610075, China.
College of Acupuncture and Moxibustion, Chengdu University of Traditional Chinese Medicine, Chengdu 610075, China.
Zhen Ci Yan Jiu. 2018 May 25;43(5):296-301. doi: 10.13702/j.1000-0607.170966.
To observe whether the transient receptor potential vanilloid 1(TRPV 1) plays a role in moxibustion (Moxi)-induced regulation of local immune inflammatory response.
Fifteen C 57 BL/6 J and 15 TRPV 1 mice were randomly and respectively divided into blank control, Moxi-30 min and Moxi-72 h groups (5 mice/group). The Moxi intervention ([46±1] ℃) was applied to the left "Zusanli" (ST 36) for 30 min. The local tissue of the left ST 36 region was collected for observing the histological changes after H．E. staining, and for counting the number of tumor necrosis factor-α (TNF-α) immune-reaction (IR)-positive cells after immunofluorescence staining.
In both C 57 BL/6 J and TRPV 1 mice, following Moxi, the local epidermis tissue was incomplete, with vague layers and arrangement of the stratum corneum (being thicker than control mice), and visible scar tissue. The corium layer was relatively looser in the structure, and the collagenous and elastic fibers were loosened in the arrangement or absence, with an abundant inflammatory cell infiltration, vascular dilation and congestion of blood vessels, and abnormal hyperplasia of hair follicles and sebaceous glands (but without marked structural changes of the subcutaneous tissue). In C 57 BL/6 J mice, compared with the control group, the numbers of TNF-α IR-positive cells were significantly increased in the epidermis of the Moxi-30 min group (<0.05), and in the dermis of the Moxi-30 min and -72 h groups (<0.01). In TRPV 1 mice, compared with the control group, the numbers of TNF-α IR-positive cells were also considerably increased in the epidermis of the Moxi-30 min group, and in the dermis of both Moxi-30 min and -72 h groups (<0.01). Comparison between the two Moxi groups showed that the number of TNF-α positive cells was significantly lower in the dermis of the Moxi-72 h group than in that of the Moxi-30 min group in C 57 BL/6 J mice (<0.01), and significantly lower in both the epidermis and dermis of the Moxi-72 h group than in those of the Moxi-30 min group in TRPV 1 mice (<0.01)．.
Moxibustion stimulation of ST 36 can induce structural changes of the regional epidermis and dermis of the skin and up-regulate the number of TNF-α IR-positive cells in both C 57 BL/6 J and TRPV 1 mice, which may contribute to its therapeutic effect via local TRPV 1-independent immunoregulation.
观察瞬时受体电位香草酸亚型1（TRPV1）在艾灸调控局部免疫炎症反应中是否发挥作用。
将15只C57BL/6J小鼠和15只TRPV1小鼠分别随机分为空白对照组、艾灸30分钟组和艾灸72小时组（每组5只）。对左侧“足三里”（ST36）进行艾灸干预（[46±1]℃），持续30分钟。采集左侧ST36区域的局部组织，进行苏木精-伊红（H.E.）染色后观察组织学变化，并进行免疫荧光染色后计数肿瘤坏死因子-α（TNF-α）免疫反应（IR）阳性细胞数量。
在C57BL/6J小鼠和TRPV1小鼠中，艾灸后局部表皮组织不完整，层次模糊，角质层排列紊乱（比对照小鼠厚），可见瘢痕组织。真皮层结构相对疏松，胶原纤维和弹性纤维排列疏松或缺失，有大量炎性细胞浸润，血管扩张、充血，毛囊和皮脂腺异常增生（但皮下组织无明显结构改变）。在C57BL/6J小鼠中，与对照组相比，艾灸30分钟组表皮中TNF-α IR阳性细胞数量显著增加（P<0.05），艾灸30分钟组和72小时组真皮中TNF-α IR阳性细胞数量显著增加（P<0.01）。在TRPV1小鼠中，与对照组相比，艾灸30分钟组表皮中TNF-α IR阳性细胞数量也显著增加，艾灸30分钟组和72小时组真皮中TNF-α IR阳性细胞数量均显著增加（P<0.01）。两组艾灸组比较显示，C57BL/6J小鼠中，艾灸72小时组真皮中TNF-α阳性细胞数量显著低于艾灸30分钟组（P<0.01）；TRPV1小鼠中，艾灸72小时组表皮和真皮中TNF-α阳性细胞数量均显著低于艾灸30分钟组（P<0.01）。
艾灸刺激ST36可诱导C57BL/6J小鼠和TRPV1小鼠皮肤局部表皮和真皮结构改变，并上调TNF-α IR阳性细胞数量，这可能是其通过局部非TRPV1依赖的免疫调节发挥治疗作用的机制。
