Laczkó-Zöld Eszter, Komlósi Andrea, Ülkei Timea, Fogarasi Erzsébet, Croitoru Mircea, Fülöp Ibolya, Domokos Erzsébet, Ştefănescu Ruxandra, Varga Erzsébet
1 Department of Pharmacognosy and Phytotherapy, University of Medicine and Pharmacy of Târgu Mureş , Gh. Marinescu nr. 38, 540139 Târgu Mureş , Romania.
2 Faculty of Pharmacy, University of Medicine and Pharmacy of Târgu Mureş , student.
Acta Biol Hung. 2018 Jun;69(2):156-169. doi: 10.1556/018.69.2018.2.5.
In this study, we analyzed extracts of Ribes (black currant, red currant and gooseberry) fruits obtained with methanol, methanol 50% and water. For each extract total polyphenol content, total flavonoid content and total anthocyanin content was assessed. The antioxidant activity of extracts was evaluated by 1,1-Diphenyl-2-picrylhydrazyl (DPPH) and 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) radical scavenging capacity and by the photo-chemiluminescence (PCL) method. Identification and quantification of individual phenolic compounds was performed by means of high performance liquid chromatograph coupled with diode array detector (HPLC-DAD) analyses. From each fruit, best extraction of polyphenols was obtained with methanol 50%. In case of red currants and gooseberry there was no significant difference in flavonoids and anthocyanins extraction rate by the different extraction solvents. For black currants the methanol and methanol 50% extract presented the highest antioxidant activity. For red currants extracts with methanol 50% showed stronger antioxidant activity (IC = 5.71 mg/ml for DPPH, IC = 1.17 mg/ml for ABTS) than those with methanol or water. In case of gooseberry by the DPPH test the water extract proved to be the most active (IC = 5.9 mg/ml). In the PCL test black currants methanol 50% extract was over 6 times more powerful as the ones from red currants. In case of gooseberries, water extract presented the highest antioxidant activity (41.84 μmol AAE/g). In black currant cyanidin-3-glucoside was the major compound. Quercetin 3-O-glucoside was identified in each sample. From cinnamic acid derivatives neochlorogenic acid was present in black currants in the highest amount (356.33 μg/g).
在本研究中，我们分析了用甲醇、50%甲醇和水提取的茶藨子（黑加仑、红加仑和醋栗）果实提取物。对每种提取物的总多酚含量、总黄酮含量和总花青素含量进行了评估。通过1,1-二苯基-2-苦基肼（DPPH）和2,2'-联氮-双-（3-乙基苯并噻唑啉-6-磺酸）（ABTS）自由基清除能力以及光化学发光（PCL）法评估提取物的抗氧化活性。通过高效液相色谱仪结合二极管阵列检测器（HPLC-DAD）分析对单个酚类化合物进行鉴定和定量。从每种果实中，用50%甲醇能最好地提取多酚。对于红加仑和醋栗，不同提取溶剂对黄酮类化合物和花青素的提取率没有显著差异。对于黑加仑，甲醇和50%甲醇提取物表现出最高的抗氧化活性。对于红加仑，50%甲醇提取物显示出比甲醇或水提取物更强的抗氧化活性（DPPH的IC = 5.71 mg/ml，ABTS的IC = 1.17 mg/ml）。在醋栗的情况下，通过DPPH测试，水提取物被证明是最具活性的（IC = 5.9 mg/ml）。在PCL测试中，黑加仑50%甲醇提取物的活性比红加仑提取物高6倍以上。对于醋栗，水提取物表现出最高的抗氧化活性（41.84 μmol AAE/g）。在黑加仑中，矢车菊素-3-葡萄糖苷是主要化合物。在每个样品中都鉴定出了槲皮素3-O-葡萄糖苷。在肉桂酸衍生物中，新绿原酸在黑加仑中的含量最高（356.33 μg/g）。
