Varga E, Domokos E, Fogarasi E, Steanesu R, Fülöp I, Croitoru M D, Laczkó-Zöld E
Acta Pharm Hung. 2017;87(1):19-25.
Prunus spinosa L. (blackthorn, sloe) is a com- mon species in the wild flora of Europe. Marmalade, syrup, and alcoholic beverages have been prepared from fruits. In folk medicine they'are used due to the astringent effect. However there are few studies on these indigenous fruits. According to the literature they contain tannins, anthocyanins, sugars, vitamin C etc.
Our objective is to determine the antioxidant activity as related to their phenolic composition. For this purpose we prepared extracts using methanol, methanol-water (1: 1) and water. The antioxidant activity was determined by DPPH method and by photochemiluminescens (PCL) method. The total polyphenols, total anthocyanins and flavonoids were determined by colorimetric methods. Individual polyphenols were identified by a RP-HPLC-UVIVIS method.
The antioxidant activity decreased in the extracts as follows: methanol > methanol-water > water (IC₅₀= 1.33 mg/ml for DPPH; 11.94 μmol AAEIml for PCL > IC₅₀ = 1.87 mg/ml for DPPH; 10.35 μmol AAElml for PCL > IC₅₀ = 15.29 mg/ml for DPPH, 1.89 μmol AAElml for PCL) which is cor- related with the total polyphenol content (369 mg/100g > 244 mg1100g > 101 mg1100g) and total anthocyanin content (37.11 mg/100 g > 16.33 mg/100g > 7.76 mg/100g). The fla- vonoid content is similar in the three extracts (between 35.82 - 37.32 mg1100 g). The HPLC analysis shows high chloro- genic and neochlorogenic acid levels, followed by glycosides of quercetin.
Our results demonstrated that blackthorn fruits are a rich source of phenolic compounds, with anti- oxidant activity, which are best extracted with methanol or methanol-water.
黑刺李是欧洲野生植物区系中的常见物种。其果实可用于制作果酱、糖浆和酒精饮料。在民间医学中，因其具有收敛作用而被使用。然而，对这些本土水果的研究较少。据文献记载，它们含有单宁、花青素、糖类、维生素C等。
我们的目标是确定与其酚类成分相关的抗氧化活性。为此，我们用甲醇、甲醇 - 水（1:1）和水制备提取物。通过DPPH法和光化学发光（PCL）法测定抗氧化活性。用比色法测定总多酚、总花青素和黄酮类化合物的含量。通过反相高效液相色谱 - 紫外/可见光谱法鉴定单个多酚。
提取物中的抗氧化活性如下降低：甲醇＞甲醇 - 水＞水（DPPH法的IC₅₀ = 1.33毫克/毫升；PCL法为11.94微摩尔AAE/毫升＞DPPH法的IC₅₀ = 1.87毫克/毫升；PCL法为10.35微摩尔AAE/毫升＞DPPH法的IC₅₀ = 15.29毫克/毫升，PCL法为1.89微摩尔AAE/毫升），这与总多酚含量（369毫克/100克＞244毫克/100克＞101毫克/100克）和总花青素含量（37.11毫克/100克＞16.33毫克/100克＞7.76毫克/100克）相关。三种提取物中的黄酮类化合物含量相似（在35.82 - 37.32毫克/100克之间）。高效液相色谱分析表明绿原酸和新绿原酸含量较高，其次是槲皮素糖苷。
我们的结果表明，黑刺李果实是富含酚类化合物的来源，具有抗氧化活性，用甲醇或甲醇 - 水提取效果最佳。
