Lin T
Endocrinology. 1985 Jul;117(1):119-26. doi: 10.1210/endo-117-1-119.
Purified rat Leydig cell cytosol was found to contain a protein kinase which was dependent on the presence of both calcium and phospholipids (phosphatidylserine and diolein), i.e. calcium/phospholipid-dependent protein kinase. The peak of Ca/phospholipid-dependent protein kinase was separated from type I and type II cAMP-dependent protein kinase by DE-52 chromatography. 4 beta-Phorbol-12-myristate-13-acetate (PMA), a tumor-promoting agent, could substitute for diolein in activation of Ca/phospholipid-dependent protein kinase. PMA caused dose-dependent increments of testosterone formation by Leydig cells, whereas inactive phorbol esters had no significant effects. PMA-induced testosterone formation was dependent on extracellular calcium and could be blocked by the addition of the calcium channel-blocking agent nifedipine. Since PMA can directly activate Ca/phospholipid-dependent protein kinase and increase testosterone formation, these results suggest that Ca/phospholipid-dependent protein kinase may be involved in modulating Leydig cell steroidogenesis in addition to the classical cAMP-dependent protein kinase pathway.
已发现纯化的大鼠睾丸间质细胞胞质溶胶含有一种蛋白激酶，该激酶依赖于钙和磷脂（磷脂酰丝氨酸和二油精）的存在，即钙/磷脂依赖性蛋白激酶。通过DE-52柱层析将钙/磷脂依赖性蛋白激酶的峰与I型和II型环磷酸腺苷依赖性蛋白激酶分离。4β-佛波醇-12-肉豆蔻酸酯-13-乙酸酯（PMA），一种促癌剂，可替代二油精激活钙/磷脂依赖性蛋白激酶。PMA导致睾丸间质细胞睾酮生成呈剂量依赖性增加，而无活性的佛波醇酯则无显著影响。PMA诱导的睾酮生成依赖于细胞外钙，并且可通过添加钙通道阻滞剂硝苯地平来阻断。由于PMA可直接激活钙/磷脂依赖性蛋白激酶并增加睾酮生成，这些结果表明，除了经典的环磷酸腺苷依赖性蛋白激酶途径外，钙/磷脂依赖性蛋白激酶可能参与调节睾丸间质细胞的类固醇生成。
