Papadopoulos V, Carreau S, Drosdowsky M A
FEBS Lett. 1985 Sep 2;188(2):312-6. doi: 10.1016/0014-5793(85)80393-8.
When the phorbol ester, 4 beta-phorbol-12-myristate-13-acetate (PMA) or bacterial phospholipase C (PL-C) is added to a preparation of purified adult rat Leydig cells, containing 2 mM CaCl2, a time- and dose-dependent decreases of LH-stimulated testosterone production is observed. After a 3 h stimulation with oLH (100 ng/ml), PMA (100 ng/ml) and PL-C (1.6 U/ml) do not affect the cell viability or the hCG specific binding, while cAMP accumulation is significantly reduced; cAMP-stimulated steroidogenesis is diminished only in the presence of PL-C. These observations suggest that in vitro: (i) activated Ca2+- and phospholipid-dependent protein kinase is implicated in the regulation of rat Leydig cell steroidogenesis by LH at a step before the adenylate cyclase; (ii) phospholipids play an important role in cAMP-stimulated testosterone synthesis.
当将佛波酯、4β-佛波醇-12-肉豆蔻酸酯-13-乙酸酯（PMA）或细菌磷脂酶C（PL-C）添加到含有2 mM氯化钙的纯化成年大鼠睾丸间质细胞制剂中时，观察到促黄体生成素（LH）刺激的睾酮生成呈时间和剂量依赖性下降。在用oLH（100 ng/ml）刺激3小时后，PMA（100 ng/ml）和PL-C（1.6 U/ml）不影响细胞活力或人绒毛膜促性腺激素（hCG）特异性结合，而环磷酸腺苷（cAMP）积累显著减少；仅在PL-C存在的情况下，cAMP刺激的类固醇生成才会减弱。这些观察结果表明，在体外：（i）激活的钙和磷脂依赖性蛋白激酶在LH调节大鼠睾丸间质细胞类固醇生成过程中，在腺苷酸环化酶之前的步骤起作用；（ii）磷脂在cAMP刺激的睾酮合成中起重要作用。
