Watanabe Louis P, Riddle Nicole C
Department of Biology, University of Alabama at Birmingham.
Department of Biology, University of Alabama at Birmingham;
J Vis Exp. 2018 May 27(135):57751. doi: 10.3791/57751.
Drosophila melanogaster is a new model organism for studies in exercise biology. To date, two main exercise systems, the Power Tower and the Treadwheel have been described. However, a method to measure the amount of additional animal activity induced through the exercise treatment has been lacking. The Rotating Exercise Quantification System (REQS) fills this need, providing a measure of animal activity for animals experiencing rotational exercise. This protocol details how to use the REQS to assess animal activity during rotational exercise and illustrates the type of data that can be generated. Here, we demonstrate how the REQS is used to measure sex- and strain-specific differences in exercise induced activity. The REQS can also be used to evaluate the impact of various other experimental parameters such as age, diet, or population size on exercise induced activity. In addition, it can be used to compare the efficacy of different exercise training protocols. Importantly, it provides an opportunity to standardize exercise treatments between strains, allowing the researcher to achieve equal amounts of activity between groups if needed. Thus, the REQS is a notable new resource for exercise biologists working with the Drosophila model system and complements existing exercise systems.
黑腹果蝇是运动生物学研究中的一种新型模式生物。迄今为止，已经描述了两种主要的运动系统，即力量塔和跑步机。然而，一直缺乏一种测量运动处理所诱导的额外动物活动量的方法。旋转运动量化系统（REQS）满足了这一需求，为经历旋转运动的动物提供了一种测量动物活动的方法。本方案详细介绍了如何使用REQS评估旋转运动期间的动物活动，并说明了可以生成的数据类型。在这里，我们展示了如何使用REQS来测量运动诱导活动中的性别和品系特异性差异。REQS还可用于评估各种其他实验参数，如年龄、饮食或种群大小对运动诱导活动的影响。此外，它可用于比较不同运动训练方案的效果。重要的是，它提供了一个在品系间标准化运动处理的机会，使研究人员能够在需要时使各组之间的活动量相等。因此，REQS是使用果蝇模型系统的运动生物学家的一个重要新资源，并补充了现有的运动系统。
