Strodel W E, Walker W A, Vinik B S, Heldsinger A, Eckhauser F E, Vinik A I
Endocrinology. 1985 Jul;117(1):376-81. doi: 10.1210/endo-117-1-376.
Stimuli originating in the small intestine enhance gastric acid secretion, an effect termed the intestinal phase of gastric secretion. A humoral agent, enterooxyntin (EO) may mediate this effect. Mechanical distension of an ex vivo-perfused segment of canine jejunum caused the release of EO measured by cytochemical quantification of hydroxyl ion production (HIP) in guinea pig gastric oxyntic cells, an index of acid secretion. The H2 receptor antagonist, cimetidine (10(-5) M), and diamine oxidase, which hydrolyzes endogenous histamine, reduced HIP by 60% and 48%, respectively. Atropine (10(-5) M), the muscarinic cholinergic antagonist, reduced HIP by 75%. EO-induced HIP was also inhibited partially by the Ca2+ antagonist EGTA (10(-6) M) and by blockade of calcium channels with LaCl3 (10(-6) M). EO does not appear to operate through any single pathway. EO may be a single substance, different from gastrin, or a mixture of substances that have stimulatory effects on the oxyntic cell. Its action on the oxyntic cell is apparently mediated by both histaminergic and cholinergic pathways. Since neither cimetidine nor atropine completely inhibited EO-induced HIP, a direct effect of EO on the oxyntic cell seems likely and appears to depend on Ca2+. Isolation and purification of EO will be necessary to better assess the potency, efficacy, and the detailed cellular mechanisms of EO action.
源自小肠的刺激会增强胃酸分泌，这种效应被称为胃酸分泌的肠期。一种体液因子，肠泌酸素（EO）可能介导这种效应。犬空肠离体灌注段的机械扩张导致了EO的释放，这是通过豚鼠胃壁细胞中氢离子产生（HIP）的细胞化学定量来测量的，HIP是酸分泌的一个指标。H2受体拮抗剂西咪替丁（10^(-5) M）和水解内源性组胺的二胺氧化酶分别使HIP降低了60%和48%。毒蕈碱型胆碱能拮抗剂阿托品（10^(-5) M）使HIP降低了75%。EO诱导的HIP也被Ca2+拮抗剂乙二醇双四乙酸（EGTA，10^(-6) M）和用氯化镧（10^(-6) M）阻断钙通道部分抑制。EO似乎不是通过任何单一途径起作用。EO可能是一种不同于胃泌素的单一物质，或者是对壁细胞有刺激作用的物质混合物。它对壁细胞的作用显然是由组胺能和胆碱能途径介导的。由于西咪替丁和阿托品都没有完全抑制EO诱导的HIP，EO对壁细胞的直接作用似乎是可能的，并且似乎依赖于Ca2+。为了更好地评估EO作用的效力、功效和详细的细胞机制，有必要对EO进行分离和纯化。
