Walker W A, Strodel W E, Eckhauser F E, Heldsinger A, Vinik A I
J Surg Res. 1983 May;34(5):486-92. doi: 10.1016/0022-4804(83)90100-2.
A humoral factor may mediate the intestinal phase of gastric acid secretion. An ex vivo perfused segment of canine jejunum maintained by an oxygenated asanguinous physiologic perfusate was used to test for release of an enterooxyntin (EO) in response to balloon distention at 30 mm Hg for 15 min. Gastric acid secretion in guinea pig fundic mucosa was determined indirectly by a quantitative cytochemical bioassay (CBA) of oxyntic cell hydroxyl ion production (HIP). An increase in the optical density (OD) caused by the cytochemical stain in the oxyntic cells reflects HIP, an index of acid secretion. Basal OD for segments with distention was 16.6 +/- 0.53 and for those without 15.5 +/- 0.68 (NS). Results are expressed as mean change of OD from basal (mean delta OD +/- SEM). (Table-see text) EO caused greater stimulation of HIP than gastrin or histamine. EO was heat stable. Trichloroacetic acid treatment decreased EO activity as did pronase digestion suggesting that EO is composed of one or more peptides.
EO, an acid secretagogue, is a humoral agent probably composed of one or more peptides and is released by small bowel distention. Mechanical distention of the small bowel may be an important mechanism for the perpetuation of gastric acid secretion. The ex vivo perfused jejunal segment in conjunction with the CBA are ideal tools with which to study mechanisms of release of EO and the mechanism of action of EO on the oxyntic cell.
一种体液因子可能介导胃酸分泌的肠期。使用由含氧无血生理灌注液维持的犬空肠离体灌注段，来检测肠泌酸素（EO）在30毫米汞柱压力下球囊扩张15分钟后是否释放。通过对壁细胞羟离子产生（HIP）的定量细胞化学生物测定法（CBA）间接测定豚鼠胃底黏膜中的胃酸分泌。壁细胞中细胞化学染色引起的光密度（OD）增加反映HIP，即酸分泌的指标。扩张段的基础OD为16.6±0.53，未扩张段为15.5±0.68（无显著性差异）。结果以OD相对于基础值的平均变化表示（平均ΔOD±标准误）。（见表文）与胃泌素或组胺相比，EO对HIP的刺激作用更强。EO对热稳定。三氯乙酸处理和链霉蛋白酶消化均降低了EO活性，提示EO由一种或多种肽组成。
EO作为一种酸分泌剂，是一种可能由一种或多种肽组成的体液因子，由小肠扩张释放。小肠的机械性扩张可能是胃酸分泌持续存在的重要机制。离体灌注空肠段与CBA相结合是研究EO释放机制及EO对壁细胞作用机制的理想工具。
