体内细胞介导免疫、血凝抑制反应、血液学和生化值在本地与外来鸡种中的比较。

In vivo cell-mediated immune, hemagglutination inhibition response, hematological and biochemical values in native vs. exotic chicken breeds.

机构信息

ICAR-Directorate of Poultry Research, Hyderabad 500030, India.

ICAR-Indian Agricultural Statistics Research Institute, India.

出版信息

Poult Sci. 2018 Sep 1;97(9):3063-3071. doi: 10.3382/ps/pey182.

DOI:10.3382/ps/pey182

PMID:29889283

Abstract

Birds (364) of both sexes, 11-wk-old, belonging to 2 native (Brown Nicobari and Ghagus) breeds and 1 exotic breed (Dahlem Red) were evaluated for cell-mediated immune response (CMI) by phytohemagglutinin-P (PHA-P), hemagglutination inhibition (HI) assay against Newcastle disease virus (NDV) antigen (LaSota stock virus), flow cytometric analysis of CD8+ cytotoxic T lymphocytes (CTLs), and hematology and biochemical assays. The cutaneous basophil hypersensitivity response PHA-P% increase in wattle thickness (mm) was highest in Ghagus (431.14 ± 22.56) which differed significantly with that of Brown Nicobari (269.1 ± 22.66) and Dahlem Red (218.42 ± 22.30). Sex-wise observation showed that females are having significantly higher response than males. Hemagglutination inhibition test was performed to determine the serum antibodies against Newcastle disease (ND) virus. Brown Nicobari showed highest HI antibody titer than Ghagus and Dahlem Red to similar vaccination program after booster NDV dose. Flow cytometry assay revealed significantly higher CTLs proliferation in Brown Nicobari than Ghagus and Dahlem Red. Moreover, CTLs were found to be higher in control group than the treatment group. Other hematological parameters (103/μL) significant difference was found in white blood cell count between Dahlem Red (38.41 ± 1.03) with that of Brown Nicobari (35.28 ± 1.04) and Ghagus (34.57 ± 1.04) in treatment groups. Same trend was observed in the Lymphocyte treatment group. However, in Granulocyte treatment group, Brown Nicobari (11.04 ± 0.35) was found to be significantly different from Dahlem Red (8.68 ± 0.34) and Ghagus (9.27 ± 0.35). Correlations between body weight at 11 wk of age and CMI, HI, cytotoxic T cell were -0.093, 0.047, and -0.036, respectively. Egg weight was found to be positively correlated with that of chick weight. Serum biochemical values showed that Dahlem Red was having significantly higher creatinine levels compared to Ghagus. Triglycerides level was also significantly higher in Ghagus compared to Dahlem Red. No significant breed effect was observed for alkaline phosphate, aspartate transaminase, and alanine transaminase. Cholesterol and total serum protein levels were significantly higher in Dahlem Red compared to Brown Nicobari.

摘要

对 364 只 11 周龄的雌雄鸟类进行了细胞介导免疫反应 (CMI) 的评价，这些鸟类属于 2 个本地品种（Brown Nicobari 和 Ghagus）和 1 个外来品种（Dahlem Red），评价方法包括植物血球凝集素-P（PHA-P）、针对新城疫病毒（NDV）抗原（LaSota 株病毒）的血凝抑制（HI）试验、CD8+细胞毒性 T 淋巴细胞（CTLs）的流式细胞分析以及血液学和生化分析。Ghagus 的鸡冠厚度 PHA-P%增加（mm）最高（431.14 ± 22.56），明显高于 Brown Nicobari（269.1 ± 22.66）和 Dahlem Red（218.42 ± 22.30）。性别观察显示，雌性的反应明显高于雄性。进行血凝抑制试验以确定针对新城疫（ND）病毒的血清抗体。Brown Nicobari 比 Ghagus 和 Dahlem Red 对类似的疫苗接种方案表现出更高的 HI 抗体滴度，并且在加强剂量 NDV 后。流式细胞术分析显示，Brown Nicobari 的 CTLs 增殖明显高于 Ghagus 和 Dahlem Red。此外，在对照组中发现 CTLs 高于治疗组。其他血液学参数（每微升 103 个），Dahlem Red（38.41 ± 1.03）与 Brown Nicobari（35.28 ± 1.04）和 Ghagus（34.57 ± 1.04）的白细胞计数有显著差异治疗组。淋巴细胞治疗组也观察到相同的趋势。然而，在粒细胞治疗组中，Brown Nicobari（11.04 ± 0.35）与 Dahlem Red（8.68 ± 0.34）和 Ghagus（9.27 ± 0.35）的差异有统计学意义。11 周龄时体重与 CMI、HI、细胞毒性 T 细胞的相关性分别为-0.093、0.047 和-0.036。鸡蛋重量与雏鸡重量呈正相关。血清生化值显示，与 Ghagus 相比，Dahlem Red 的肌酐水平明显较高。Ghagus 的甘油三酯水平也明显高于 Dahlem Red。碱性磷酸酶、天冬氨酸转氨酶和丙氨酸转氨酶没有观察到明显的品种效应。胆固醇和总血清蛋白水平在 Dahlem Red 中明显高于 Brown Nicobari。