通过二维核Overhauser增强光谱法研究酵母苯丙氨酸转运核糖核酸的亚氨基质子共振。

Imino-proton resonances of yeast tRNAPhe studied by two-dimensional nuclear Overhauser enhancement spectroscopy.

作者信息

Heerschap A, Mellema J R, Janssen H G, Walters J A, Haasnoot C A, Hilbers C W

出版信息

Eur J Biochem. 1985 Jun 18;149(3):649-55. doi: 10.1111/j.1432-1033.1985.tb08973.x.

DOI:10.1111/j.1432-1033.1985.tb08973.x

Abstract

Application of two-dimensional nuclear Overhauser enhancement (NOE) spectroscopy to yeast tRNAPhe in H2O solution demonstrates that all imino-proton resonances, related to the secondary structure, and nearly all imino proton resonances, originating from the tertiary structure, can be assigned efficiently by this method. The results corroborate the assignments of the imino-proton resonances of this tRNA as established previously by one-dimensional NOE experiments (only the assignment of base pairs G1 X C72 and C2 X G71 should be reversed). The advantages of two-dimensional NOE spectroscopy over one-dimensional NOE spectroscopy for the assignments of imino-proton resonances and the structure elucidation of tRNA are illustrated and discussed. Furthermore, the use of non-exchangeable proton resonances as probes of the molecular structure is explored.

摘要

二维核Overhauser增强（NOE）光谱在H2O溶液中对酵母苯丙氨酸转运RNA（tRNAPhe）的应用表明，所有与二级结构相关的亚氨基质子共振以及几乎所有源自三级结构的亚氨基质子共振，都可以通过该方法有效地进行归属。结果证实了先前通过一维NOE实验确定的该转运RNA亚氨基质子共振的归属（仅碱基对G1 X C72和C2 X G71的归属应颠倒）。阐述并讨论了二维NOE光谱相对于一维NOE光谱在亚氨基质子共振归属和转运RNA结构解析方面的优势。此外，还探索了使用非交换质子共振作为分子结构探针的方法。