特定氘代大分子中的核Overhauser效应：用于转运RNA中异常碱基配对的核磁共振分析
Nuclear Overhauser effect in specifically deuterated macromolecules: NMR assay for unusual base pairing in transfer RNA.
作者信息
Sánchez V, Redfield A G, Johnston P D, Tropp J
出版信息
Proc Natl Acad Sci U S A. 1980 Oct;77(10):5659-62. doi: 10.1073/pnas.77.10.5659.
Abstract
We demonstrate a fairly general method for identification of NMR absorption lines of macromolecues extracted from microorganisms, based on nuclear Overhauser effects (NOE). Several NOE in tRNA are observable between resolved imino proton resonances and ring carbon resonances that are either C(2) protons of adenine or C(8) protons of adenine or guanine. Yeast tRNAPhe was deuterated at the purine C(8) positins by heating in 2H2O and also biosynthetically. NOE between imino protons and adenine C(2) protons of standard A . U base pairs would not be affected by such a label, but some other NOE that might be otherwise similar, such as those of reverse Hoogsteen base pairs, should disappear. Six NOE were shown to be from standard A . U pairs by their nondisappearance. Four NOE from methyl resonances to aromatic proton resonances did disappear. The results disagree with previous assignments based on ring-current theories of imino proton NMR shifts.
摘要
我们展示了一种相当通用的方法，用于识别从微生物中提取的大分子的核磁共振吸收线，该方法基于核Overhauser效应（NOE）。在分辨出的亚氨基质子共振与环碳共振之间，可以观察到tRNA中的几种NOE，这些环碳共振要么是腺嘌呤的C(2)质子，要么是腺嘌呤或鸟嘌呤的C(8)质子。酵母苯丙氨酸tRNA通过在2H2O中加热以及生物合成的方式，在嘌呤C(8)位置进行了氘代。标准A.U碱基对的亚氨基质子与腺嘌呤C(2)质子之间的NOE不会受到这种标记的影响，但其他一些可能类似的NOE，如反向Hoogsteen碱基对的NOE，应该会消失。通过六个NOE未消失，表明它们来自标准A.U对。从甲基共振到芳族质子共振的四个NOE确实消失了。这些结果与先前基于亚氨基质子核磁共振位移的环流理论所做的归属不一致。
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