Laboratory for Stem Cell Research, Aalborg University, Fredrik Bajers Vej 3B, 9220, Aalborg, Denmark.
Sir Alan Parks Physiology Unit, St. Mark's Hospital, Northwick Park, Watford Road, Harrow, HA1 3UJ, United Kingdom.
Biochem Biophys Res Commun. 2018 Sep 3;503(1):271-277. doi: 10.1016/j.bbrc.2018.06.014. Epub 2018 Jun 15.
This study explored the feasibility of constructing a tissue engineered muscle layer in the oesophagus using oesophageal acellular matrix (OAM) scaffolds and human aortic smooth muscle cells (hASMCs) or human adipose-derived stem cells (hASCs). The second objective was to investigate the effect of hypoxic preconditioning of seeding cells on cell viability and migration depth. Our results demonstrated that hASMCs and hASCs could attach and adhere to the decellularized OAM scaffold and survive and proliferate for at least 7 days depending on the growth conditions. This indicates adipose-derived stem cells (ASCs) have the potential to substitute for smooth muscle cells (SMCs) in the construction of tissue engineered oesophageal muscle layers.
本研究探索了使用食管脱细胞基质（OAM）支架和人主动脉平滑肌细胞（hASMCs）或人脂肪来源干细胞（hASCs）构建食管组织工程肌肉层的可行性。第二个目标是研究种子细胞缺氧预处理对细胞活力和迁移深度的影响。我们的结果表明，hASMCs 和 hASCs 可以附着并黏附在脱细胞化的 OAM 支架上，并根据生长条件在至少 7 天内存活和增殖。这表明脂肪来源干细胞（ASCs）有可能替代平滑肌细胞（SMCs）用于构建组织工程食管肌肉层。
