College of Animal Science and Veterinary Medicine, Henan Institute of Science and Technology, Xinxiang, Henan, 453003, PR China.
College of Animal Science and Veterinary Medicine, Henan Institute of Science and Technology, Xinxiang, Henan, 453003, PR China.
Biochem Biophys Res Commun. 2018 Nov 30;506(3):765-771. doi: 10.1016/j.bbrc.2018.06.028. Epub 2018 Jun 23.
The regulatory role of long non-coding RNAs (lncRNAs) in various biological functions has been demonstrated. However, their role in fat deposition and lipid metabolism in pigs remains less understood. To explore the expression profile of lncRNAs in Jinhua and Landrace pigs, we investigated the expression levels of lncRNAs in intramuscular adipose tissues obtained from these pigs. Results showed that the expression levels of lncRNAs in these pig breeds significantly (Fold Change ≥ 2.0, FDR < 0.05) differed. A total of 4910 lncRNAs were identified, and 119 of these lncRNAs were differentially expressed. Of these differentially expressed lncRNAs, 60 and 59 were up- and down-regulated, respectively. The differentially expressed lncRNAs are involved in mitogen-activated protein kinase (MAPK) signaling pathway, Ras signaling pathway, PI3k-Akt signaling pathway. We then compared these differentially expressed lncRNAs with mRNAs and found that six of the co-expressed lncRNAs were implicated in pathways related to fat deposition and lipid metabolism. Overall, our results revealed a remarkable difference in fat metabolism in intramuscular adipose tissues of pigs, and provide a basis for subsequent research on fat deposition.
长链非编码 RNA（lncRNA）在各种生物功能中的调节作用已得到证实。然而，它们在猪脂肪沉积和脂质代谢中的作用仍知之甚少。为了研究 lncRNA 在金华猪和长白猪中的表达谱，我们研究了从这些猪的肌内脂肪组织中获得的 lncRNA 的表达水平。结果表明，这些猪品种的 lncRNA 表达水平存在显著差异（倍数变化≥2.0， FDR<0.05）。共鉴定出 4910 个 lncRNA，其中 119 个 lncRNA差异表达。这些差异表达的 lncRNA 参与丝裂原活化蛋白激酶（MAPK）信号通路、Ras 信号通路、PI3k-Akt 信号通路。然后，我们将这些差异表达的 lncRNA 与 mRNAs 进行比较，发现其中 6 个共表达的 lncRNA 与脂肪沉积和脂质代谢相关途径有关。总之，我们的研究结果揭示了猪肌内脂肪组织中脂肪代谢的显著差异，为后续脂肪沉积研究提供了依据。
