Crites B R, Vishwanath R, Arnett A M, Bridges P J, Burris W R, McLeod K R, Anderson L H
Department of Animal and Food Sciences, University of Kentucky, Lexington, KY 40546, United States.
Sexing Technologies, Navasota, TX 77868, United States.
Theriogenology. 2018 Sep 15;118:126-129. doi: 10.1016/j.theriogenology.2018.05.003. Epub 2018 Jun 6.
The objective of this study was to compare conception rates of female beef cattle inseminated at a fixed-time with either conventional (CON) or SexedUltra™ sex-sorted (SU) semen. Treatments included CON or SU with two sires represented within each treatment. Cows (n = 316) and heifers (n = 78) from six locations were randomly assigned treatment. Ovulation was synchronized in all females using the industry-standard 7-d CO-Synch + controlled internal drug release (CIDR) protocol (100 μg GnRH + CIDR [1.38 g progesterone] on d 0, 25 mg PGF2α at CIDR removal on d 7, and 100 μg GnRH on d 10, 54 h (heifers) or 60 h (cows) after CIDR removal). Estrotect™ estrous detection aids were applied at CIDR removal and patch activation was recorded at insemination. Animals were assumed estrual if greater than 50% of the patch coating was removed. The results from this study indicated no main effects of treatment (P = 0.82), sire (P = 0.64), or age (P=0.8) on AI conception rates. Additionally, there were not significant interactions between sire and treatment (P=0.19) or age and treatment (P=0.29). There was however, a significant (P=0.0005) effect of estrous expression on conception rates. Conception rate for estrual females (62.8%) was greater (p=0.0001) than non-estrual females (38.7%) at FTAI regardless of treatment. Furthermore, the conception rates were similar (P = 0.61) between conventional (61.9%) and sex-sorted semen (63.8%) when estrus was expressed prior to FTAI. Larger studies are warranted to determine appropriate timing of insemination with sex-sorted semen in FTAI protocols to maximize pregnancy potential.
本研究的目的是比较在固定时间用常规(CON)或性别超选(SU)精液人工授精的雌性肉牛的受孕率。处理包括CON或SU,每种处理中有两个公牛代表。来自六个地点的母牛(n = 316)和小母牛(n = 78)被随机分配处理。使用行业标准的7天CO-Synch + 控制内部药物释放(CIDR)方案使所有雌性动物排卵同步(第0天注射100μg促性腺激素释放激素 + CIDR [1.38g孕酮],第7天取出CIDR时注射25mg前列腺素F2α,第10天注射100μg促性腺激素释放激素,在取出CIDR后54小时(小母牛)或60小时(母牛))。在取出CIDR时应用Estrotect™发情检测辅助工具,并在人工授精时记录贴片激活情况。如果超过50%的贴片涂层被去除,则假定动物处于发情期。本研究结果表明,处理(P = 0.82)、公牛(P = 0.64)或年龄(P = 0.8)对人工授精受孕率没有主要影响。此外,公牛与处理(P = 0.19)或年龄与处理(P = 0.29)之间没有显著相互作用。然而,发情表现对受孕率有显著(P = 0.0005)影响。无论处理如何,发情期雌性动物在定时人工授精时的受孕率(62.8%)高于未发情雌性动物(38.7%)(P = 0.0001)。此外,当在定时人工授精前表现出发情时,常规精液(61.9%)和性别分选精液(63.8%)的受孕率相似(P = 0.61)。需要进行更大规模的研究,以确定在定时人工授精方案中使用性别分选精液的合适授精时间,以最大限度地提高怀孕潜力。