富集含Y精子并结合定时人工授精以获得西门塔尔杂交雄性后代。
The enriched Y-bearing sperm combined with delayed fixed-time artificial insemination for obtaining male Simmental crossbred offspring.
作者信息
Meles Dewa Ketut, Mustofa Imam, Hariadi Mas'ud, Wurlina Wurlina, Susilowati Suherni, Amaliya Anny, Suparto Suparto, Rimayanti Rimayanti
机构信息
Division of Basic Veterinary Medicine, Faculty of Veterinary Medicine, Universitas Airlangga, Surabaya, Kampus C Mulyorejo, Surabaya 601155, East Java, Indonesia.
Division of Veterinary Reproduction, Faculty of Veterinary Medicine, Universitas Airlangga, Surabaya, Kampus C Mulyorejo, Surabaya 601155, East Java, Indonesia.
出版信息
Vet World. 2022 Jan;15(1):102-109. doi: 10.14202/vetworld.2022.102-109. Epub 2022 Jan 22.
BACKGROUND AND AIM
The production of male calf beef cattle is an agricultural innovation needed to increase the farm's productivity as a provider of meat sources. This study aimed to determine the sex ratio of the offspring of cows inseminated with Y-bearing sperm enriched by Percoll density gradient centrifugation and swim-up, combined with delayed fixed-time artificial insemination (FTAI).
MATERIALS AND METHODS
Ejaculates of Simmental bulls were divided into four equal portions and grouped as T0 (control, non-sexed semen), T1 and T2 were sexed semen using Percoll density gradient centrifugation three and five levels, respectively, and T3 was sexed semen using swim-up. After the sex was sorted, the semen was diluted in a tris-egg yolk extender, packaged in French mini-straws containing 50 million live sperm cells, and frozen. Pre-sexed, post-sexed, and post-thawed spermatozoa were evaluated based on progressive motility, viability, intact plasma membrane, and abnormality. The post-thawed semen of T0 was artificially inseminated to recipient cows at 12 h after onset of estrus (not delayed FTAI). Meanwhile, the delayed FTAI was conducted 18-20 h after onset of estrus using the T0, the best of T1 and T2, and the T3 post-thawed semen.
RESULTS
The Percoll density gradient centrifugation reduced motility, viability, and intact plasma membrane but increased sperm abnormalities. Meanwhile, the swim-up process increased motility, viability, and intact plasma membrane of sperm cells but decreased sperm abnormalities. Post-thawed semen decreased motility, viability, and intact plasma membrane of sperm cells but increased sperm abnormalities. The sex ratio of the Simmental crossbred offspring was 96.08% and 100% in T1 and T3, respectively, compared to 48.25% and 67.39% in T0 not delayed and delayed FTAI, respectively.
CONCLUSION
The Percoll density gradient centrifugation and swim-up methods are prospective for obtaining male offspring.
背景与目的
生产雄性犊牛肉牛是提高农场作为肉类来源提供者生产力所需的一项农业创新。本研究旨在确定经Percoll密度梯度离心和上浮法富集含Y精子并结合延迟定时人工授精（FTAI）的母牛所产后代的性别比例。
材料与方法
西门塔尔公牛的精液分为四个相等部分并分组，T0（对照，未分选精液），T1和T2分别使用三级和五级Percoll密度梯度离心法进行性别分选精液，T3使用上浮法进行性别分选精液。性别分选后，精液在Tris-蛋黄稀释液中稀释，包装在含有5000万个活精子细胞的法国微型细管中，并冷冻。对分选前、分选后和解冻后的精子进行了渐进运动性、活力、完整质膜和异常情况的评估。T0解冻后的精液在发情开始后12小时对受体母牛进行人工授精（非延迟FTAI）。同时，使用T0、T1和T2中最佳的以及T3解冻后的精液在发情开始后18 - 20小时进行延迟FTAI。
结果
Percoll密度梯度离心降低了精子的运动性、活力和完整质膜，但增加了精子异常率。同时，上浮过程增加了精子细胞的运动性、活力和完整质膜，但降低了精子异常率。解冻后的精液降低了精子细胞的运动性、活力和完整质膜，但增加了精子异常率。西门塔尔杂交后代的性别比例在T1和T3中分别为96.08%和100%，而在T0非延迟和延迟FTAI中分别为48.25%和67.39%。
结论
Percoll密度梯度离心法和上浮法在获得雄性后代方面具有前景。
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