The Key Lab of Pollution Control and Ecosystem Restoration in Industry Clusters, Ministry of Education, School of Environment and Energy, South China University of Technology, Guangzhou Higher Education Mega Centre, Guangzhou 510006, PR China.
The Key Lab of Pollution Control and Ecosystem Restoration in Industry Clusters, Ministry of Education, School of Environment and Energy, South China University of Technology, Guangzhou Higher Education Mega Centre, Guangzhou 510006, PR China.
Ecotoxicol Environ Saf. 2018 Oct;161:356-363. doi: 10.1016/j.ecoenv.2018.06.011. Epub 2018 Jun 8.
This study constructed a biological-inorganic hybrid system including Pseudomonas putida (P. putida) and bioreduced Pd (0) nanoparticles (NPs), and inspected the influence of bio-nano Pd (0) on the direct electron transfer and phenol biodegradation. Scanning electron microscopy and energy-dispersive X-ray spectroscopy (SEM-EDX) showed that bio-nano Pd (0) (~10 nm) were evenly dispersed on the surface and in the periplasm of P. putida. With the incorporation of bio-nano Pd (0), the redox currents of bacteria in the cyclic voltammetry (CV) became higher and the oxidation current increased as the addition of lactate, while the highest increase rates of two electron transfer system (ETS) rates were 63.97% and 33.79%, respectively. These results indicated that bio-nano Pd (0) could directly promote the electron transfer of P. putida. In phenol biodegradation process, P. putida-Pd (0)- 2 showed the highest k (0.2992 h), μ (0.035 h) and K (714.29 mg/L) and the lowest apparent K (76.39 mg/L). The results of kinetic analysis indicated that bio-nano Pd (0) markedly enhanced the biocatalytic efficiency, substrate affinity and the growth of cells compared to native P. putida. The positive effects of bio-nano Pd (0) to the electron transfer of P. putida would promote the biodegradation of phenol.
本研究构建了一个包含恶臭假单胞菌（Pseudomonas putida）和生物还原钯（0）纳米颗粒（NPs）的生物-无机杂化系统，并考察了生物-纳米 Pd（0）对直接电子转移和苯酚生物降解的影响。扫描电子显微镜和能谱（SEM-EDX）显示，生物-纳米 Pd（0）（~10nm）均匀分散在 P. putida 的表面和周质中。随着生物-纳米 Pd（0）的加入，细菌在循环伏安法（CV）中的氧化还原电流变得更高，并且随着乳酸盐的加入，氧化电流增加，而两个电子转移系统（ETS）速率的最高增加率分别为 63.97%和 33.79%。这些结果表明，生物-纳米 Pd（0）可以直接促进 P. putida 的电子转移。在苯酚生物降解过程中，P. putida-Pd（0）-2 表现出最高的 k（0.2992 h）、μ（0.035 h）和 K（714.29 mg/L），以及最低的表观 K（76.39 mg/L）。动力学分析的结果表明，与原生 P. putida 相比，生物-纳米 Pd（0）显著提高了生物催化效率、底物亲和力和细胞的生长。生物-纳米 Pd（0）对 P. putida 电子转移的积极影响会促进苯酚的生物降解。
