University of Toronto, Hospital of Sick Children, Toronto, Canada.
Emory University, Department of Biology, Atlanta, Georgia, USA.
Mol Cells. 2018 Jul 31;41(7):613-621. doi: 10.14348/molcells.2018.0143. Epub 2018 Jun 12.
The capacity of differentiation of human pluripotent stem cells (hPSCs), which include both embryonic stem cells and induced pluripotent stem cells, into cardiomyocytes (CMs) provides an unlimited resource for human CMs for a wide range of applications such as cell based cardiac repair, cardiac drug toxicology screening, and human cardiac disease modeling. However, their applicability is significantly limited by immature phenotypes. It has been well known that currently available CMs derived from hPSCs (hPSC-CMs) represent immature embryonic or fetal stage CMs and are functionally and structurally different from mature human CMs. To overcome this critical issue, several new approaches aiming to generate more mature hPSC-CMs have been developed. This review describes recent approaches to generate more mature hPSC-CMs including their scientific principles, advantages, and limitations.
人类多能干细胞（hPSCs），包括胚胎干细胞和诱导多能干细胞，向心肌细胞（CMs）分化的能力为广泛的应用提供了无限的人类 CMs 资源，例如基于细胞的心脏修复、心脏药物毒理学筛选和人类心脏疾病建模。然而，其适用性受到不成熟表型的显著限制。众所周知，目前从 hPSCs 中获得的心肌细胞（hPSC-CMs）代表不成熟的胚胎或胎儿期的 CMs，在功能和结构上与成熟的人类 CMs 不同。为了克服这个关键问题，已经开发了几种旨在生成更成熟的 hPSC-CMs 的新方法。本综述描述了生成更成熟的 hPSC-CMs 的最新方法，包括其科学原理、优点和局限性。
