使用流式细胞术检测细胞内 IgD 可能是诊断 IgD 多发性骨髓瘤的一种新颖且补充的方法。

Detection of intracellular IgD using flow cytometry could be a novel and supplementary method to diagnose IgD multiple myeloma.

机构信息

Hematology Department, China-Japan Friendship Hospital, Ying-Hua-Yuan East Street, No. 2, Beijing, 100029, China.

出版信息

BMC Cancer. 2018 Jun 11;18(1):650. doi: 10.1186/s12885-018-4562-8.

DOI:10.1186/s12885-018-4562-8

PMID:29890944

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5996556/

Abstract

BACKGROUND

We examined whether detecting the heavy chain of cytoplasmic immunoglobulin D (IgD) by flow cytometry could be used as a supplemental method to diagnose IgD multiple myeloma (MM).

METHODS

Bone marrow (BM) samples of thirty-five patients with MM were collected. Five of them were IgD MM, the rest of thirty were other subtypes of MM. Antibodies to four types of heavy chains of immunoglobulin (e.g., IgA, IgG, IgM, and IgD) were analyzed by flow cytometry in each patient's BM sample.

RESULTS

The five IgD MM patients were all positive for cytoplasmic IgD. The percentage of IgD positive MM cells among nucleated cells varied from 0.4 to 12.9%. Cytoplasmic IgG was positive in eight patients with IgG MM (n = 9); cytoplasmic IgA was positive in all patients with IgA MM (n = 10); cytoplasmic IgM was positive in one patient with IgM MM (n = 1). No heavy chain was detected in light chain MM (n = 9) and non-secretory subtype (n = 1).

CONCLUSIONS

Detection of cytoplasmic IgD by flow cytometry is a convenient, sensitive and supplemental method to diagnose IgD MM.

摘要

背景

我们研究了通过流式细胞术检测免疫球蛋白 D（IgD）重链是否可作为诊断 IgD 多发性骨髓瘤（MM）的辅助方法。

方法

采集 35 例 MM 患者的骨髓（BM）样本。其中 5 例为 IgD MM，其余 30 例为其他 MM 亚型。对每位患者 BM 样本中的四种免疫球蛋白重链（如 IgA、IgG、IgM 和 IgD）进行流式细胞术分析。

结果

5 例 IgD MM 患者均为细胞质 IgD 阳性。核细胞中 IgD 阳性 MM 细胞的百分比为 0.4%至 12.9%。8 例 IgG MM 患者的细胞质 IgG 阳性（n=9）；10 例 IgA MM 患者的细胞质 IgA 均阳性（n=10）；1 例 IgM MM 患者的细胞质 IgM 阳性（n=1）。轻链 MM（n=9）和非分泌型（n=1）未检测到重链。