鼠伤寒沙门氏菌LT2的araBAD操纵子。I. araB的核苷酸序列及其产物核糖激酶的一级结构。

The araBAD operon of Salmonella typhimurium LT2. I. Nucleotide sequence of araB and primary structure of its product, ribulokinase.

作者信息

Lin H C, Lei S P, Wilcox G

出版信息

Gene. 1985;34(1):111-22. doi: 10.1016/0378-1119(85)90301-4.

Abstract

Hybrid plasmids containing the araBAD operon of Salmonella typhimurium LT2 were characterized by Southern blot and genetic analyses. The nucleotide sequence of araB was determined. The araB gene product, ribulokinase (EC 2.7.1.16), was purified and the results of amino acid composition analysis and partial amino acid sequence are in agreement with predictions from the DNA sequence. Ribulokinase is 569 amino acid residues long and has a calculated Mr of 61 793. Ribulokinase shares significant homology with xylulose kinase from Escherichia coli. Codon usage in the araB gene does not favor those codons which have intermediate codon-anticodon binding energy.

摘要

通过Southern印迹法和遗传分析对含有鼠伤寒沙门氏菌LT2的araBAD操纵子的杂交质粒进行了表征。测定了araB的核苷酸序列。纯化了araB基因产物核糖激酶（EC 2.7.1.16），氨基酸组成分析和部分氨基酸序列的结果与DNA序列的预测一致。核糖激酶长569个氨基酸残基，计算的分子量为61793。核糖激酶与大肠杆菌的木酮糖激酶具有显著的同源性。araB基因中的密码子使用不倾向于那些具有中等密码子-反密码子结合能的密码子。