编码大鼠肝脏微粒体UDP-葡糖醛酸基转移酶的cDNA的克隆
Cloning of cDNAs coding for rat hepatic microsomal UDP-glucuronyltransferases.
作者信息
Jackson M R, McCarthy L R, Corser R B, Barr G C, Burchell B
出版信息
Gene. 1985;34(2-3):147-53. doi: 10.1016/0378-1119(85)90122-2.
Radioiodinated, affinity-purified, anti-UDP-glucuronyltransferase (UDPGT) antibodies have been used to isolate cDNAs coding for UDPGT(s) from a rat liver cDNA library cloned in the expression vector bacteriophage lambda gt11. The sizes of ten cloned cDNAs range from 0.3-2.1 kb. The identity of the cDNAs was confirmed by the hybrid-select translation and immunochemical analyses. Restriction mapping indicates that two classes of cDNA coding for different UDPGT mRNAs have been cloned.
放射性碘化的、亲和纯化的抗UDP-葡糖醛酸基转移酶（UDPGT）抗体已被用于从克隆于表达载体噬菌体λgt11的大鼠肝脏cDNA文库中分离编码UDPGT的cDNA。十个克隆的cDNA大小范围为0.3 - 2.1 kb。通过杂交选择翻译和免疫化学分析证实了这些cDNA的身份。限制性图谱分析表明已克隆出两类编码不同UDPGT mRNA的cDNA。
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