Laboratoire d'Ecologie Microbienne, Faculté des Sciences de la Nature et de la Vie, Université de Bejaia, 06000 Bejaia, Algeria.
CIRAD, Univ. Montpellier, IRD, INRA, SupAgro, LSTM, Montpellier, France.
Syst Appl Microbiol. 2018 Sep;41(5):452-459. doi: 10.1016/j.syapm.2018.05.005. Epub 2018 May 31.
Fifty-two slow-growing strains were isolated from root nodules of Calicotome spinosa grown in the Northeast of Algeria and grouped in 24 rep-PCR clusters. One representative strain for each profile was further phylogenetically characterized. The nearly complete 16S rRNA gene sequence indicated that all strains were affiliated to Bradyrhizobium. Multi-Locus Sequence Analysis (MLSA) of the atpD, glnII and recA genes and of the 16S-23S rRNA internal transcribed spacer (ITS) showed that these strains formed four divergent clusters: one close to Bradyrhizobium canariense and Bradyrhizobium lupini and three others separate from all the described species, representing three putative new Bradyrhizobium species. A phylogenetic analysis based on the nodC gene sequence affiliated the strains to either of the two symbiovars, genistearum or retamae.
从阿尔及利亚东北部生长的 Calicotome spinosa 根瘤中分离出 52 株生长缓慢的菌株，并将其分为 24 个 rep-PCR 聚类。对每个图谱的一个代表菌株进行了进一步的系统发育特征分析。近完整的 16S rRNA 基因序列表明，所有菌株均属于慢生根瘤菌属。atpD、glnII 和 recA 基因以及 16S-23S rRNA 内部转录间隔区（ITS）的多位点序列分析（MLSA）表明，这些菌株形成了四个不同的聚类：一个与 Bradyrhizobium canariense 和 Bradyrhizobium lupini 密切相关，另外三个与所有描述的物种分离，代表了三个潜在的新慢生根瘤菌属物种。基于 nodC 基因序列的系统发育分析将菌株分为 genistearum 或 retamae 两个共生型。
