Bai Hongzhi, Zhang Yanhua, Yu Haiqiu, Irfan Muhammad, Huang Yuqian, Han Mei, Yang Jinfeng, Liu Ning, Wang Hui, Han Xiaori
College of Land and Environment, Shenyang Agricultural University, Shenyang, China.
College of Agronomy, Shenyang Agricultural University, Shenyang, China.
Mol Biol Res Commun. 2019 Jun;8(2):59-68. doi: 10.22099/mbrc.2019.32983.1392.
L is a legume of economic importance, which is nodulated by a slow-growing bacteria. However there is no well characterization of this rhizobia in many areas of China. In the present study, cross-inoculation experiments were performed in cowpea and soybean. The isolated bacteria strains were characterized physiologyically, biochemically and identified through 16S rDNA sequence analysis showing that it belongs to . The genetic diversity of the seventeen isolated strains were assessed through PCR-RFLP of 16S rDNA and 16S-23S rDNA IGS region. Cross inoculation test indicated that isolates could nodulate cowpea but not soybean. The cluster analysis based on physiological and biochemical characteristics showed the lower correlation between isolates and sites. The isolates were grouped into four clusters based on 16S rDNA gene sequence analysis. Thirteen polymorphisms were variable across all observations in 16S rDNA RFLP and six different IGS types from isolates. The results implies that there was some association between geographical factor and phylogenetic diversity of indigenous isolates.
L是一种具有经济重要性的豆科植物，由一种生长缓慢的细菌形成根瘤。然而，在中国许多地区，这种根瘤菌尚未得到充分的表征。在本研究中，对豇豆和大豆进行了交叉接种实验。对分离出的细菌菌株进行了生理生化特征分析，并通过16S rDNA序列分析进行鉴定，结果表明其属于 。通过对16S rDNA和16S - 23S rDNA IGS区域进行PCR - RFLP分析，评估了17株分离菌株的遗传多样性。交叉接种试验表明，分离株能够使豇豆结瘤，但不能使大豆结瘤。基于生理生化特征的聚类分析表明，分离株与位点之间的相关性较低。基于16S rDNA基因序列分析，分离株被分为四个簇。在16S rDNA RFLP的所有观察结果中，有13个多态性是可变的，分离株有6种不同的IGS类型。结果表明，地理因素与本地分离株的系统发育多样性之间存在一定关联。
