Martin B, Prats H, Claverys J P
Gene. 1985;34(2-3):293-303. doi: 10.1016/0378-1119(85)90138-6.
The hexA mismatch repair gene of Streptococcus pneumoniae has been cloned into multicopy plasmid vectors. The cloned hexA gene is expressed as judged from its ability to complement various chromosomal hexA- alleles. Its direction of transcription was defined and the functional limits were localized by original methods relying on homology-dependent integration of nonautonomous chimeric plasmids carrying chromosomal inserts into the chromosome. Comparison of the proteins encoded by recombinant plasmids and by restriction fragments allowed us to identify an Mr 94 000 protein as the probable product of the hexA gene.
肺炎链球菌的hexA错配修复基因已被克隆到多拷贝质粒载体中。从其互补各种染色体hexA-等位基因的能力判断，克隆的hexA基因是有表达的。通过依靠携带染色体插入片段的非自主嵌合质粒同源依赖性整合到染色体中的原始方法，确定了其转录方向并定位了功能界限。对重组质粒和限制性片段所编码蛋白质的比较，使我们能够鉴定出一种94000 Mr的蛋白质可能是hexA基因的产物。
