Laboratory for the Improvement of Plants and Valorization of Agroressources, National School of Engineering of Sfax (ENIS), University of Sfax, Sfax 3038, Tunisia.
Department of Life Sciences, University of Modena and Reggio Emilia, Modena, Italy.
Carbohydr Polym. 2018 Sep 15;196:272-278. doi: 10.1016/j.carbpol.2018.05.019. Epub 2018 May 17.
In this study, chondroitin sulfate/dermatan sulfate was isolated and purified from the skin of corb (Sciaena umbra) (CSG) with a yield of 6.2%. Chemical and structural analysis showed that CSG consisted of high sulfate content 28.74% and an average molecular weight of 15.46 KDa. The separation of CSG by agarose-gel electrophoresis revealed the presence of DS and CS. Structural analysis of the purified CS/DS by means of SAX-HPLC after treatment with specific chondroitinases showed that this polymer was composed of nonsulfated disaccharide, monosulfated disaccharides and disulfated disaccharides in various percentages. The results also suggest that the percentage of CS and DS recovred in CSG were 24% and 76%, respectively. Anticoagulant activity in vitro was measured in plasma using classical anticoagulation tests: activated partial thromboplastin time (aPTT), thrombin time (TT) and prothrombine time (PT) tests. The findings thus indicated that the purified CS/DS exhibits a remarkably high anticoagulant effect.
在这项研究中，从 Corb（Sciaena umbra）的皮肤中分离和纯化了硫酸软骨素/硫酸皮肤素（CSG），得率为 6.2%。化学和结构分析表明，CSG 由高硫酸含量 28.74%和平均分子量 15.46 kDa 组成。琼脂糖凝胶电泳分离 CSG 显示 DS 和 CS 的存在。用特定的软骨素酶处理后，通过 SAX-HPLC 对纯化的 CS/DS 的结构分析表明，该聚合物由不同比例的非硫酸化二糖、单硫酸化二糖和二硫酸化二糖组成。结果还表明，CSG 中回收的 CS 和 DS 的百分比分别为 24%和 76%。在血浆中使用经典抗凝试验：活化部分凝血活酶时间（aPTT）、凝血酶时间（TT）和凝血酶原时间（PT）试验测量体外抗凝活性。因此，研究结果表明，纯化的 CS/DS 具有显著的抗凝作用。
