Nicklas J A, Noreen H J, Segall M, Bach F H
Hum Immunol. 1985 Jun;13(2):95-103. doi: 10.1016/0198-8859(85)90016-3.
DNA from individuals of four Dw subtypes of DR4 (Dw4, Dw10, Dw14, Dw15) were studied using Southern blotting to determine if subtype-specific DR beta or DQ beta restriction fragment polymorphism could be found. Although very little polymorphism was found among ten DR4 homozygous individuals (4 Dw4, 2 Dw10, 3 Dw14, 1 Dw15) using a Dr beta or a DQ beta probe, restriction fragment polymorphism was easily detected between different DR types (DR1-DRw8). The possible evolutionary significance of the lack of Dw-associated polymorphism relative to DR-associated polymorphism is discussed.
运用Southern印迹法对DR4的四种Dw亚型（Dw4、Dw10、Dw14、Dw15）个体的DNA进行研究，以确定是否能找到亚型特异性的DRβ或DQβ限制性片段多态性。尽管使用DRβ或DQβ探针在10名DR4纯合个体（4名Dw4、2名Dw10、3名Dw14、1名Dw15）中发现的多态性极少，但在不同的DR类型（DR1-DRw8）之间很容易检测到限制性片段多态性。本文讨论了相对于DR相关多态性而言，缺乏Dw相关多态性可能具有的进化意义。
