Chen Jian-Woon, Lau Yin Yin, Krishnan Thiba, Chan Kok-Gan, Chang Chien-Yi
International Genome Centre, Jiangsu University, Zhenjiang, China.
Division of Genetics and Molecular Biology, Institute of Biological Sciences, Faculty of Science, University of Malaya, Kuala Lumpur, Malaysia.
Front Microbiol. 2018 May 28;9:1104. doi: 10.3389/fmicb.2018.01104. eCollection 2018.
is a rod-shaped Gram-negative bacterium which is notably known as a pathogen in humans, animals, and plants. Infections caused by especially in hospitalized patients are often life-threatening and rapidly increasing worldwide throughout the years. Recently, multidrug-resistant has taken a toll on humans' health due to the inefficiency of antimicrobial agents. Therefore, the rapid and advanced diagnostic techniques to accurately detect this bacterium particularly in clinical samples are indeed necessary to ensure timely and effective treatments and to prevent outbreaks. This review aims to discuss most recent of state-of-the-art molecular diagnostic techniques enabling fast and accurate detection and identification of based on well-developed genotyping techniques, e.g., polymerase chain reaction, pulse-field gel electrophoresis, and next generation sequencing. The advantages and limitations of each of the methods are also reviewed.
是一种杆状革兰氏阴性菌，作为人类、动物和植物中的病原体而闻名。尤其是在住院患者中由其引起的感染往往危及生命，并且多年来在全球范围内迅速增加。近年来，由于抗菌药物的低效性，多重耐药的[细菌名称]对人类健康造成了损害。因此，特别是在临床样本中准确检测这种细菌的快速先进诊断技术对于确保及时有效的治疗和预防疫情爆发确实是必要的。本综述旨在讨论基于成熟的基因分型技术（如聚合酶链反应、脉冲场凝胶电泳和下一代测序）能够快速准确地检测和鉴定[细菌名称]的最新先进分子诊断技术。还对每种方法的优点和局限性进行了综述。
