Wendt Mitchell, Heo Gang-Joon
Veterinary Medical Center and College of Veterinary Medicine, Chungbuk National University, Cheongju, Korea.
Lab Anim Res. 2016 Dec;32(4):208-216. doi: 10.5625/lar.2016.32.4.208. Epub 2016 Dec 23.
Our research sought to characterize the phylogeny of isolated from pet Chinese stripe-necked turtles () to better understand its evolutionary relation to other isolates and increase understanding of a potential zoonotic pathogen transmitted through direct contact with pet turtles. Thirty-one isolates were obtained from both immature and adult turtles sold in pet shops in Korea. To characterize the phylogenic position of Chinese stripe-necked turtle-borne relative to other strains, multilocus sequence typing (MLST) analysis was performed due to the accessibility and breadth of MLST databases. Seven housekeeping genes (, , , , , , and ) were sequenced and the results were compared with data from the MLST database. The genes were further used for phylogenetic analysis of using concatenated gene fragments. Both rooted and unrooted phylogenetic trees were generated. Eleven distinct sequence types were present within the isolates among which seven were new. Expanding an unrooted phylogenetic tree to include MLST sequences isolated from various other geographic locations and sources revealed a divergent cluster containing the majority of isolates obtained from turtles. This suggests that strains particularly well-adapted for inhabiting turtles occupy a distinct phylogenetic position.
我们的研究旨在对从宠物中华条颈龟（）中分离出的[病原体名称未给出]的系统发育进行表征，以便更好地了解其与其他分离株的进化关系，并增进对通过直接接触宠物龟传播的潜在人畜共患病原体的认识。从韩国宠物店出售的未成年和成年龟中获得了31株[病原体名称未给出]分离株。为了表征中华条颈龟携带的[病原体名称未给出]相对于其他菌株的系统发育位置，由于多位点序列分型（MLST）数据库的可及性和广度，进行了MLST分析。对七个管家基因（[基因名称未给出]、[基因名称未给出]、[基因名称未给出]、[基因名称未给出]、[基因名称未给出]、[基因名称未给出]和[基因名称未给出]）进行了测序，并将结果与MLST数据库中的数据进行了比较。这些基因进一步用于使用串联基因片段对[病原体名称未给出]进行系统发育分析。生成了有根和无根的系统发育树。分离株中存在11种不同的序列类型，其中7种是新的。将无根系统发育树扩展以纳入从其他不同地理位置和来源分离的[病原体名称未给出]的MLST序列，发现了一个包含从龟中获得的大多数分离株的不同聚类。这表明特别适应于在龟体内生存的[病原体名称未给出]菌株占据了一个独特的系统发育位置。
