大肠杆菌中对阿米洛利敏感的钠氢逆向转运蛋白
Amiloride-sensitive Na+-H+ antiporter in Escherichia coli.
作者信息
Mochizuki-Oda N, Oosawa F
出版信息
J Bacteriol. 1985 Jul;163(1):395-7. doi: 10.1128/jb.163.1.395-397.1985.
Abstract
In everted vesicles of Escherichia coli, delta pH caused by H+ efflux through the Na+/H+ antiporter was measured by using a fluorescent dye. Amiloride inhibited the activity of the Na+/H+ antiporter. Kinetic studies showed that amiloride competed with Na+. The inhibition constant of 40 microM was obtained.
摘要
在大肠杆菌的外翻囊泡中，利用荧光染料测定了通过Na⁺/H⁺反向转运体排出H⁺所引起的ΔpH。氨氯吡咪抑制了Na⁺/H⁺反向转运体的活性。动力学研究表明，氨氯吡咪与Na⁺存在竞争。获得了40微摩尔的抑制常数。
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