Arias I M, Forgac M
J Biol Chem. 1984 May 10;259(9):5406-8.
ATP-dependent trapping of [14C]methylamine was demonstrated in vesicles selectively derived from the sinusoidal plasma membrane of rat hepatocytes; activity was lacking in vesicles prepared from the canalicular domain of the plasma membrane of rat hepatocytes. The proton movement was inhibited by carbonyl cyanide p-trifluoromethoxyphenylhydrazone, strophanthidin, vanadate, amiloride, and absence of sodium. 22Na efflux from sinusoidal membrane vesicles increased inversely to extravesicular pH. The results indicate that the sinusoidal plasma membrane of rat hepatocytes contains a Na+/H+ antiport.
在从大鼠肝细胞窦状质膜选择性提取的囊泡中证实了ATP依赖的[14C]甲胺捕获；而从大鼠肝细胞质膜胆小管区域制备的囊泡中则缺乏该活性。质子移动受到羰基氰化物对三氟甲氧基苯腙、毒毛花苷G、钒酸盐、氨氯吡脒的抑制，且在无钠的情况下也受到抑制。来自窦状膜囊泡的22Na外流与囊泡外pH呈反比增加。结果表明，大鼠肝细胞的窦状质膜含有Na+/H+逆向转运体。
