评估小鼠下丘脑-垂体-甲状腺（HPT）轴

Evaluating the Hypothalamic-Pituitary-Thyroid (HPT) Axis in Mice.

作者信息

Wondisford F E

机构信息

Department of Medicine, Rutgers-Robert Wood Johnson Medical School, Henry Rutgers Term Chair and Chancellor Scholar Rutgers, The State University of New Jersey, New Brunswick, NJ, 08901, USA.

出版信息

Methods Mol Biol. 2018;1801:155-161. doi: 10.1007/978-1-4939-7902-8_13.

DOI:10.1007/978-1-4939-7902-8_13

PMID:29892823

Abstract

As the genome of experimental animals has become easier to manipulate, a number of mouse models have been developed to understand in vivo thyroid hormone action. A major site of thyroid hormone action is the HPT axis. While several methods are available that provide a detailed understanding of the HPT axis in mice, many authors choose to include only cursory data about this axis, which can lead to erroneous conclusions about in vivo thyroid hormone action. A standard protocol is proposed to evaluate the HPT axis in mice.

摘要

随着实验动物基因组变得更容易操控，人们开发了许多小鼠模型来了解甲状腺激素在体内的作用。甲状腺激素作用的一个主要部位是下丘脑-垂体-甲状腺（HPT）轴。虽然有几种方法可用于详细了解小鼠的HPT轴，但许多作者选择只纳入关于该轴的粗略数据，这可能导致关于甲状腺激素在体内作用的错误结论。本文提出了一个评估小鼠HPT轴的标准方案。