Wu Shijia, Dellinger Julien, Markossian Suzy, Dusabyinema Yves, Guyot Romain, Hughes Sandrine, Aubert Denise, Fackeure Marie, Gauthier Karine, Gillet Benjamin, Jiang Wenzheng, Flamant Frédéric
Institut de Génomique Fonctionnelle de Lyon, UMR5242, École Normale Supérieure de Lyon, INRAE USC1370, CNRS, Lyon 69007, France.
Shanghai Key Laboratory of Regulatory Biology, Institute of Biomedical Sciences and School of Life Sciences, East China Normal University, Dongchuan Road 500, Shanghai 200241, China.
Endocrinology. 2025 Apr 22;166(6). doi: 10.1210/endocr/bqaf084.
We present an atlas of genes that respond to thyroid hormone in the adult mouse mediobasal hypothalamus. Based on droplet-based single nuclei RNA-seq method and batch transcriptome analyses, the atlas lists putative target genes of the hormone nuclear receptors in 20 different types of neuronal and glial cells. The transcriptional regulation of these genes varies extensively across neuronal and glial cell types. However, while astrocytes appear to be highly sensitive to thyroid hormone stimulation, differentiated oligodendrocytes are relatively insensitive. This atlas is expected to promote future investigations into the molecular and cellular mechanisms that underlie the numerous functions of thyroid hormone in the hypothalamic circuits.
我们展示了成年小鼠中脑基底下丘脑对甲状腺激素产生反应的基因图谱。基于液滴式单核RNA测序方法和批量转录组分析，该图谱列出了20种不同类型神经元和神经胶质细胞中激素核受体的推定靶基因。这些基因的转录调控在神经元和神经胶质细胞类型中差异很大。然而，虽然星形胶质细胞似乎对甲状腺激素刺激高度敏感，但分化的少突胶质细胞相对不敏感。预计该图谱将促进对甲状腺激素在下丘脑回路中众多功能的分子和细胞机制的未来研究。
