Marelli Federica, Persani Luca
Department of Clinical Sciences and Community Health, University of Milan, Milan, Italy.
Lab of Endocrine and Metabolic Research, IRCCS Istituto Auxologico Italiano, Milan, Italy.
Methods Mol Biol. 2018;1801:287-298. doi: 10.1007/978-1-4939-7902-8_22.
In recent years, the zebrafish has become a powerful model not only for the developmental biology studies, but also for genetic analyses and drug screenings, mostly thanks to the ease with which its embryos can be manipulated and to its translucent body, which allows in vivo imaging. In this chapter, we will provide an overview of the current knowledge about the role of thyroid hormone receptors during zebrafish embryonic development. Moreover, we will explore the methodologies applied to zebrafish biology to knock down a gene of interest and to analyze in vivo the molecular mechanisms of the mutated receptors.
近年来，斑马鱼已成为一种强大的模型，不仅用于发育生物学研究，还用于基因分析和药物筛选，这主要归功于其胚胎易于操作以及其透明的身体，便于进行体内成像。在本章中，我们将概述目前关于甲状腺激素受体在斑马鱼胚胎发育过程中作用的知识。此外，我们将探讨应用于斑马鱼生物学的方法，以敲低感兴趣的基因并在体内分析突变受体的分子机制。
